Addgene

We narrowed to 15,233 results for: LEA

Showing: 5841 - 5860 of 15233 results

Results per page:

Sort results by:

pNHT7::RPL15::sGluc

Plasmid

#186769

Purpose

An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.011

Plasmid

#187211

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.012

Plasmid

#187212

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.015

Plasmid

#187216

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.016

Plasmid

#187217

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCT-TGC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCA-TGC])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.136

Plasmid

#187220

Purpose

Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)

Use

Tags

Expression

Bacterial

Mutation

Promoter

A: pSalTTC; B: pTet*

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBK.010

Plasmid

#187210

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-T Easy-Cas9-nls-CDS1

Plasmid

#160231

Purpose

Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

Depositor

Matias Kirst

Article

Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

Insert

Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1

Use

Level 0 of moclo golden gate position cds1

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL86977OD:PITG_15278a

Plasmid

#173545

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_15278_P17777

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHcRed-NPMMLF1nes-C1

Plasmid

#131849

Purpose

Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Use

Tags

HcRed

Expression

Mammalian

Mutation

This is a control deletion contruct of the cytop…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasper-pNos-MCP-mCherry

Plasmid

#180275

Purpose

to epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

MCP-mCherry

Use

Tags

mCherry

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y

Plasmid

#148075

Purpose

Insect Expression of DmPAN2-F1103A-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-F1103A-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y

Plasmid

#148046

Purpose

Bacterial Expression of DmPAN2-K1124EY1127EK1131E-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

Plasmid

#178871

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCCR4_A

Plasmid

#145894

Purpose

Insect Expression of DmCCR4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmCCR4 (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV V5-Tmem209

Plasmid

#175154

Purpose

Lentiviral expression of V5-tagged mouse Tmem209

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem209 (Tmem209 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV Zdhhc6-V5

Plasmid

#175146

Purpose

Lentiviral expression of mouse Zdhhc6-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Zdhhc6 (Zdhhc6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV Ptdss2-V5

Plasmid

#175143

Purpose

Lentiviral expression of mouse Ptdss2-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Ptdss2 (Ptdss2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL86977OD:PITG_14371

Plasmid

#173567

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_14371_T30-4

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge