Addgene

We narrowed to 18,518 results for: omp

Showing: 5821 - 5840 of 18518 results

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pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

Plasmid

#130816

Purpose

Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

bJun-NFAST-NLS-DEVDG-mCherry-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-V5-B/BRISBANE/60/2008

Plasmid

#187923

Purpose

Expresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor HRas25

Plasmid

#26637

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor-HRas25 (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Use

Tags

PQS1

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBFP (GB0025)

Plasmid

#68193

Purpose

Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

BFP coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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RanBP2-RB3-4-pET23a

Plasmid

#85769

Purpose

bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

fragment (amino acids 2304-3062)

Promoter

T7

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-GRM1-IRES-CyRFP1

Plasmid

#195355

Purpose

mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

GRM1-IRES-CyRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO FAAA)3

Plasmid

#130703

Purpose

Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4

Use

Tags

Expression

Mammalian

Mutation

FKVK sequence in each SUMO1 module changed to FAAA

Promoter

CMV

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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F-Cdk8

Plasmid

#15366

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al Trends Biochem Sci. 2005 May . 30(5):250

Insert

CDK8 (CDK8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Use

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

Plasmid

#199347

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-3C

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript SK +/- mTRF1

Plasmid

#12303

Depositor

Titia de Lange

Article

Broccoli et al Hum Mol Genet. 1997 Jan . 6(1):69-76.

Insert

mTRF1 (Terf1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-PhoCl-mCherry-myc

Plasmid

#87692

Purpose

Express PhoCl-mCherry with C-term myc tag in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl-mCherry-myc

Use

Tags

myc tag

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDJSVT86

Plasmid

#108965

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

Depositor

Daniel Slade

Article

Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

Type

Empty backbone

Use

Tags

OmpA 1-27, 6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-LBR-EGFP-polyA

Plasmid

#59748

Purpose

Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

LBR-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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