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Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_mic
Plasmid#184916PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_zeo
Plasmid#184917PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 T179V
Plasmid#26997DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT179VPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NES
Plasmid#118940PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_POU5F1_P2A_Hygro_Barcode
Plasmid#120474PurposeBarcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertPOU5F1 (POU5F1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVENUS-N1-FNTA
Plasmid#86859PurposeTo express FNTA with a Venus tag at C terminus.DepositorInsertFNTA (FNTA Human)
UseTagsVenusExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
T/brachyury(2A-Dre)
Plasmid#140761PurposeTargeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.DepositorInsertDre
UseMouse TargetingTagsExpressionMutationPromoterEndogenous T/brachyuryAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN397 (FuGW-S(E2F1)p-Sponge7)
Plasmid#105181PurposeModule 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(E2F1)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shPrc1
Plasmid#160959PurposePrc1 shRNA in pMKO.1 retroviral vectorDepositorInsertPrc1 shRNA (Prc1 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I
Plasmid#146553PurposeMammalian Expression of HsSmg6-del36-576-siRNAresDepositorInsertHsSmg6-del36-576-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1
Plasmid#22420DepositorInsertArgonaut-1, variant A (AGO1 Fly)
UseDrosophila expressionTagslambdaN-HAExpressionMutationPromoterAvailable sinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S)
Plasmid#66872PurposeExpresses WTsoybean lipoxygenase-1 160S with N-term HistagDepositorInsertsoybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
UseTagsHistag from pET30Xa-LICExpressionBacterialMutationnonePromoterT7lacAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
TET-pLKO.1 PURO shId2 #2
Plasmid#83090PurposeLentiviral shRNA vector for inducible knockdown of mouse Id2DepositorInsertshId2 (Id2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-mNanog-mut-pgk-hph
Plasmid#74914PurposeMammalian expression of mNanog (Y42E, K43T)DepositorInsertNanog (Nanog Mouse)
UseTagsExpressionMammalianMutationK43T of Nanog protein and Y42E of Nanog homeodoma…PromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT9750-BEAR-mCherry
Plasmid#162990PurposeBEAR target plasmid with split mCherry and disrupted 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pRC/CMV
Plasmid#8514DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionMammalianMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSL1180 EACMCV DNA-B 1.6mer
Plasmid#159137PurposeInfectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 2007 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only