Addgene

We narrowed to 23,060 results for: GFP

Showing: 5801 - 5820 of 23060 results

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pCDNA3_PKA-Cα(K8,9A)-mEGFP

Plasmid

#168490

Purpose

Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF1-GFP

Plasmid

#67390

Purpose

Mammalian expression of hArf1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCUPI NPD-GFP

Plasmid

#1191

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 (SUP35 Budding Yeast)

Use

Cup1 promoter

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pAG414GAL-EGFP-ccdB

Plasmid

#14311

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pSIF-H1-hpolb-copGFP

Plasmid

#23256

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta shRNA

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mutation

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
Nsp13 deltaN1-EGFP

Plasmid

#165127

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp13 deltaN2-EGFP

Plasmid

#165128

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAG306GAL-ccdB-EGFP

Plasmid

#14187

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-4xGFP KI

Plasmid

#183445

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 4xGFP donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt(R25C)

Plasmid

#39532

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (Akt1 Mouse)

Use

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

R25C

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
CCR5-SZ190b-sfGFP

Plasmid

#162447

Purpose

Expression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentration

Depositor

Shuguang Zhang

Article

Qing et al iScience. 2020 Oct 28;23(12):101670. doi

Insert

C-C chemokine receptor type 5 (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

truncation from aa88 to aa249

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PKM-IRES-hrGFP

Plasmid

#73405

Purpose

Mammalian expression of mouse PKM and hrGFP

Depositor

Raymond Swanson

Article

Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

Insert

PKM (Prkcz Mouse)

Use

Tags

3xFLAG and pIRES-hrGFP-2a

Expression

Mammalian

Mutation

truncated, constitutively active form

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only
pRetroX GFP Mps1 DK

Plasmid

#63703

Purpose

Inducible expression of GFP-Mps1 DK fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 47-156, kinetochore binding domain

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV6-SMAC-HA-eGFP

Plasmid

#67492

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Use

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP K45E

Plasmid

#21734

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Mutation of the ATP-binding site (K45E). This ver…

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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1xTRE DREaMR 3p3GFP

Plasmid

#182203

Purpose

1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-GFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

Plasmid

#160634

Purpose

TU for wtGFP expression driven by 2x35s promoter.

Depositor

Diego Orzaez

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

2x35s

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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