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Plasmid#168490PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF1-GFP
Plasmid#67390PurposeMammalian expression of hArf1 with C-term GFP tagDepositorInsertARF1 (ARF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCUPI NPD-GFP
Plasmid#1191DepositorInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-EGFP-ccdB
Plasmid#14311DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSIF-H1-hpolb-copGFP
Plasmid#23256DepositorInsertDNA polymerase beta shRNA
UseLentiviral and RNAiTagsGFPExpressionMutationPromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
Nsp13 deltaN1-EGFP
Plasmid#165127Purposemammalian expression and localizationDepositorInsertNsp13 deltaN1 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nsp13 deltaN2-EGFP
Plasmid#165128Purposemammalian expression and localizationDepositorInsertNsp13 deltaN2 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-EGFP
Plasmid#14187DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Tubb3-4xGFP KI
Plasmid#183445PurposeGFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)DepositorInsertgRNA and 4xGFP donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(R25C)
Plasmid#39532DepositorInsertAkt(R25C) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationR25CPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
CCR5-SZ190b-sfGFP
Plasmid#162447PurposeExpression in HEK293T cell and compete ligand singaling against full-length receptors, the truncated receptor can perform signaling at high ligand concentrationDepositorInsertC-C chemokine receptor type 5 (CCR5 Human)
UseTagsExpressionMammalianMutationtruncation from aa88 to aa249PromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PKM-IRES-hrGFP
Plasmid#73405PurposeMammalian expression of mouse PKM and hrGFPDepositorInsertPKM (Prkcz Mouse)
UseTags3xFLAG and pIRES-hrGFP-2aExpressionMammalianMutationtruncated, constitutively active formPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRetroX GFP Mps1 DK
Plasmid#63703PurposeInducible expression of GFP-Mps1 DK fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationdeletion of aa 47-156, kinetochore binding domainPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-SMAC-HA-eGFP
Plasmid#67492PurposeTargets HA-eGFP to mitochondrial intermembrane space (IMS)DepositorInsertSMAC (DIABLO Human)
UseTagsHA-eGFPExpressionMammalianMutationaa1-59Promoterattenuated CMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAP4K3-GFP K45E
Plasmid#21734DepositorInsertMAP4K3 (MAP4K3 Human)
UseTagsGFPExpressionMammalianMutationMutation of the ATP-binding site (K45E). This ver…PromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pORANGE CACNG8-GFP KI #1
Plasmid#131473PurposeEndogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)DepositorInsertgRNA and GFP donor (Cacng8 Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-BarcodeV3
Plasmid#78631PurposeGESTALT V3 integrating barcode with Puro and EGFPDepositorInsertGESTALT lineage tracing target V3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-EGFP-BarcodeV4
Plasmid#78630PurposeGESTALT V4 integrating barcode with Puro and EGFPDepositorInsertGESTALT lineage tracing target V4
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3GFP
Plasmid#182203Purpose1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)
Plasmid#160634PurposeTU for wtGFP expression driven by 2x35s promoter.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter2x35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only