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Plasmid#124923PurposeFor expression of a.a.2-29 deletion mutant of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
UseTagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-Cep123-C2 domain
Plasmid#73329PurposeEncodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFPDepositorInsertCep123 (CEP89 Human)
UseTagsEGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS16-iGFPx6
Plasmid#105275PurposePlasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.DepositorInsertVps16 portion (VPS16 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(flex0)
Plasmid#163429PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIBA3-SpyCatcherEQ-sfGFP
Plasmid#107421PurposeFor production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.DepositorInsertSpyCatcher
UseTagsHis tag, StrepII, and superfolder GFPExpressionBacterialMutationmutation of glutamate 77 to glutaminePromoterTetAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-EGFP-ccdB
Plasmid#14309DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GSG1l-GFP KI
Plasmid#131492PurposeEndogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)DepositorInsertgRNA and GFP donor (Gsg1l Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS316-GFP-COF1
Plasmid#31843DepositorInsertCofilin (COF1 Budding Yeast)
UseTagsGFP (S65T)ExpressionYeastMutationPromoterT7Available sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
Trip-GFP-hOCLN-ΔN
Plasmid#32792DepositorInsertOCLN (OCLN Human)
UseLentiviralTagsGFPExpressionMammalianMutationDeletion of aa 1-63PromoterAvailable sinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-J23101-GFPuv
Plasmid#106390PurposepBBR6k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterLlacO-1Available sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
hRAD18 dC2-EGFP
Plasmid#68826PurposeExpresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFPDepositorInserthuman RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12a-2AeGFP
Plasmid#123633PurposeMammalian expression, Genome editingDepositorInsertBsCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(flex2)
Plasmid#163431PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mlcR25-mEGFP
Plasmid#154295PurposeFluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)DepositorInsert25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
GET1_pcDNA6.2/EmGFP-Bsd
Plasmid#176985PurposeMammalian expression vector encoding GET1 and EmGFP-BsdDepositorInsertGET1 (GET1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
7xNLS-pMJ915v2-sfGFP
Plasmid#88922PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-eGFP-HF
Plasmid#157735Purposemammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoterDepositorInserteGFP-HF
UseTagsHis8-FlagExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_AE
Plasmid#148577PurposeMammalian Expression of HsGIGYF2DepositorInsertHsGIGYF2 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(R982A)
Plasmid#39487DepositorInsertARAP3(R982A) (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationR982A = Rho GAP mutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS DV DNM1-EGFP
Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only