Addgene

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pDONR223 GABARAPL2 WT

Plasmid

#123086

Purpose

Gateway entry clone encoding human GABARAPL2 wild-type

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 HCoV-NL63 E_stop

Plasmid

#168684

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-NL63 E_nostop

Plasmid

#168666

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 LC3A G120A

Plasmid

#123070

Purpose

Gateway entry clone encoding human MAP1LC3A G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP G116A

Plasmid

#123081

Purpose

Gateway entry clone encoding human GABARAP G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 116 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120A

Plasmid

#123073

Purpose

Gateway entry clone encoding human MAP1LC3B G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP no-stop

Plasmid

#123209

Purpose

Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C no-stop

Plasmid

#123208

Purpose

Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C G126

Plasmid

#123079

Purpose

Gateway entry clone encoding human MAP1LC3C G126

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 G116A

Plasmid

#123084

Purpose

Gateway entry clone encoding human GABARAPL1 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 116 to Alanine

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Use

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP G116

Plasmid

#123082

Purpose

Gateway entry clone encoding human GABARAP G116

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP1D1T2

Plasmid

#97328

Purpose

Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

Depositor

Michael Marty

Article

Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

Insert

MSP1D1T2

Use

Tags

6xHis - TEV

Expression

Bacterial

Mutation

Addition of two T residues after T25

Promoter

T7

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 HCoV-229E N_stop

Plasmid

#168768

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

N (N )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223 HCoV-229E NSP6_stop

Plasmid

#168753

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (HCoV229Egp1 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223 HCoV-229E NSP7_stop

Plasmid

#168754

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP7 (HCoV229Egp1 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223 HCoV-229E NSP8_stop

Plasmid

#168755

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP8 (HCoV229Egp1 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223 HCoV-229E NSP10_stop

Plasmid

#168757

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP10 (HCoV229Egp1 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits
pDONR223 HCoV-229E NSP12_stop

Plasmid

#168758

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP12 (HCoV229Egp1 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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