-
Plasmid#221081PurposeSEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRs
Plasmid#67929PurposeTranslational reporter - control UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTR
Plasmid#67930PurposeTranslational reporter - Emi1 UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR
Plasmid#67932PurposeTranslational reporter - Lox 5'UTRDepositorInsertsfGFP-NLS-P2A-NLS-mCherry (LOX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR
Plasmid#67931PurposeTranslational reporter - ARHGAP5 UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E
Plasmid#113847PurposeHistone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)DepositorInsertH2B-sfGFP-P2A-tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)
Plasmid#224497PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-tdiRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)
Plasmid#224496PurposeGateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)DepositorInsertActin-Vhh-sfGFP-P2A-HA-iRFP-caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)
Plasmid#209081PurposeNgn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expressionDepositorUseTagssfGFPExpressionMammalianMutationA53T, NAC deletion (aa60-95)PromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)
Plasmid#48287DepositorTypeEmpty backboneUseTagsHis6 and msfGFPExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140230PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFPDepositorInsertLuciferase + crIFNg (reverse orientation)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor
Plasmid#211888PurposeCENP-T[1-242] transgene integration at AAVS1 safe harbor locusDepositorInsertCENP-T[1-242] (CENPT Synthetic, Human)
UseCRISPRTagsExpressionMutationT11A, T85APromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
C321.Ub-UAG-sfGFP
Bacterial Strain#98564PurposeRecoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporterDepositorBacterial ResistanceAmpicillinAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)
Plasmid#55221DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA
Plasmid#183027PurposeB6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy numberDepositorInsertH2B-sfGFP-T2A-tdTomato
UseCre/Lox and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons
Plasmid#172480PurposeH2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin geneDepositorInsertH2B-sfGFP-T2A-tdTomato
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C321.ΔClpS.Ub-UAG-sfGFP
Bacterial Strain#98565PurposeRecoded strain C321.ΔA (MutS inactivated) with genomically integrated Ubiquitin-UAG-sfGFP reporter and ClpS inactivatedDepositorBacterial ResistanceAmpicillinAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP272
Plasmid#159305PurposeYeast tagging vector to create gene fusion with sfGFP under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
C3m
Plasmid#120956PurposeMoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only