Addgene

We narrowed to 588 results for: en1

Showing: 561 - 580 of 588 results

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pCMV-PE2-P2A-hMLH1dn

Plasmid

#174827

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-hMLH1dn

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-2xSTREP-CDKN1B-Puro

Plasmid

#172611

Purpose

Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

FLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-W105M-IRES-ZsGreen

Plasmid

#68859

Purpose

Expresses Human CDK19 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1B_WT

Plasmid

#81836

Purpose

Gateway Donor vector containing CDKN1B ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1B (CDKN1B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V109G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G129E

Plasmid

#81689

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G129E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.H123Y

Plasmid

#82815

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

H123Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.R173H

Plasmid

#81682

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R173H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 1)

Plasmid

#99558

Purpose

mammalian expression of TMEM230 (isoform 1) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1B_p.C29F

Plasmid

#81307

Purpose

Gateway Donor vector containing CDKN1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1B (CDKN1B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

C29F, V109G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.K6N

Plasmid

#81698

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K6N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.F90S

Plasmid

#81693

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F90S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G127V

Plasmid

#81683

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G127V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G129V

Plasmid

#81685

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G129V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.G127R

Plasmid

#81650

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G127R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.R233Q

Plasmid

#81655

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R233Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.M35I

Plasmid

#82818

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M35I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C2_13

Plasmid

#60289

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SPATA19 inactive enhancer (SPATA19 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C2_13

Plasmid

#60252

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SPATA19 inactive enhancer (SPATA19 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-BRSK2

Plasmid

#23432

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BRSK2 (BRSK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only