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Plasmid#176061PurposeExpresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.DepositorInsertCoV-2 nsp1 (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB124 (Bcl-2 from -1287 to -3934)
Plasmid#15240DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -3934 with P1PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
LB334 (Bcl-2 from -1287 to -1640)
Plasmid#15241DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -1640 with P1PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
LB360 (Bcl-2 from -1287 to -1337)
Plasmid#15242DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -1337 with P1PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
dRMCE Plasmids
Plasmid Kit#1000000015PurposeTakes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous lociDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
P-del (pTransluc-miR-145-2)
Plasmid#21500DepositorInsert0.9 kb 5' of miR-145 (MIR145 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-ETN-lox2272
Plasmid#154833PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanerceptDepositorInsertETN
UseCre/LoxTagsExpressionMammalianMutationPromoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-lox2272
Plasmid#154828PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietinDepositorInsertcoEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO human shRNA 2 c-MAF
Plasmid#29433DepositorInsertc-MAF shRNA 2 (MAF Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro p53 shRNA 2
Plasmid#10672DepositorInsertp53 shRNA (TP53 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (fl)
Plasmid#137820Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull lengthPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 prodomain-VC155
Plasmid#49261PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBIFC-flag-hCaspase 2 prodomain-VN173
Plasmid#49262PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 prodomain (CASP2 Human)
UseTagsflag and split Venus NExpressionMammalianMutationdelete aa 148-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-HA-hCaspase 2 CARD-VC155
Plasmid#49259PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertCaspase-2 CARD (CASP2 Human)
UseTagsHA and split Venus CExpressionMammalianMutationdeleted aa 123-435PromoterCMVAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-flag-hCaspase 2 CARD-VN173
Plasmid#49260PurposeExpresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.DepositorInsertcaspase-2 CARD (CASP2 Human)
UseTagsFlag and split Venus NExpressionMammalianMutationdelete aa 123-435PromoterCMVAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v8-10 (fl)
Plasmid#137823Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v8-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull lengthPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (-cyt)
Plasmid#137821Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6-10 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic regionPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v8-10 (-cyt)
Plasmid#137822Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v8-10 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic regionPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v2-10 (fl)
Plasmid#137824Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v2-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length and mutations A282T, T483A, N535D, S5…PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v2-10 (-cyt)
Plasmid#137825Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v2-10 (-cyt) (CD44 Human)
UseTagsGFPExpressionMammalianMutationwithout cytoplasmic region - N213S on the CD44 re…PromoterPGKAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only