Addgene

We narrowed to 1,084 results for: KO

Showing: 561 - 580 of 1084 results

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pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W

Plasmid

#105018

Purpose

Lentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pggt1b (Pggt1b Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

Plasmid

#105020

Purpose

Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Znrf1 (Znrf1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21

Plasmid

#78782

Purpose

To overexpress p21 in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/CD9-GFP

Plasmid

#104402

Purpose

Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

Depositor

Jens Gruber

Article

Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

Insert

CD9 (CD9 Human)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

Plasmid

#105036

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA AKT1

Plasmid

#78778

Purpose

To overexpress AKT1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

AKT1 (AKT1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3 basic Mut E2

Plasmid

#48748

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)

Use

Luciferase

Tags

Expression

Mutation

Mutated E-box2 sequence from CACGTT to GCTAGT

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGL3 Pro Mut E2

Plasmid

#48750

Purpose

Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)

Depositor

Joseph Takahashi

Article

Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

Insert

mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)

Use

Luciferase

Tags

Expression

Mutation

Mutated E-box2 sequence from CACGTT to GCTAGT

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-puro p21

Plasmid

#78783

Purpose

Retroviral expression of p21

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mCebpe

Plasmid

#79076

Purpose

Expression of mouse Cebpe

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp epsilon (Cebpe Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
p.UTA.2.0 miR-27a-3p 3 perfect copies.

Plasmid

#82439

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Use

Tags

Expression

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

Plasmid

#105027

Purpose

Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stat3 (Stat3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

Plasmid

#105035

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

Plasmid

#105022

Purpose

Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Apc (Apc Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

Plasmid

#105017

Purpose

Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Kcmf1 (Kcmf1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K163R

Plasmid

#78787

Purpose

To overexpress p21 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K163R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K161R

Plasmid

#78788

Purpose

To overexpress p21 K161R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K161R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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hPLD3-sgRNA-Cas9_mcherry

Plasmid

#199342

Purpose

encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

Depositor

David Nemazee

Insert

hSpCas9

Use

CRISPR

Tags

P2A-RFP

Expression

Mammalian

Mutation

Promoter

human U6

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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hPLD4-sgRNA-Cas9_mcherry

Plasmid

#199343

Purpose

encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

Depositor

David Nemazee

Insert

hSpCas9

Use

CRISPR

Tags

P2A-mCherry

Expression

Mammalian

Mutation

sgRNA: accagtagtatgaagccacg

Promoter

human U6

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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