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Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorInsertLAMP1-TORCAR (LAMP1 Human, Synthetic)
UseTagsCerulean, Full-length LAMP1, and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-TORCAR
Plasmid#64930PurposePlasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertLyn-TORCAR (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR-NLS (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AI-CN590
Plasmid#62596PurposeKCTD13 N-terminus disruption by homolgy-directed repairDepositorInsertCerulean-P2A-PuroR (KCTD13 Human)
UseTALEN; Homology-directed repair donorTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-RFP-UCR1C
Plasmid#67582PurposeExpresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cellsDepositorInsertC-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)
UseTagsFLAG and TagRFPExpressionMammalianMutationAmino acid residues 80-136 of PDE4D3PromoterAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCer-TopBP1
Plasmid#211412Purposemammalian expression of human TopBP1 N-terminally fused to mCeruleanDepositorInsertTopBP1 (TOPBP1 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-RecQL4
Plasmid#211400Purposemammalian expression of human RecQL4 N-terminally fused to mCeruleanDepositorInsertRecQL4 (RECQL4 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-C16orf59
Plasmid#211422Purposemammalian expression of human C16orf59 N-terminally fused to mCeruleanDepositorInsertC16orf59 (C16orf59 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-DHX9
Plasmid#211408Purposemammalian expression of human DHX9 N-terminally fused to mCeruleanDepositorInsertDHX9 (DHX9 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-Mcm5
Plasmid#211391Purposemammalian expression of human Mcm5 N-terminally fused to mCeruleanDepositorInsertMcm5 (MCM5 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-C17orf53
Plasmid#211418Purposemammalian expression of human C17orf53 N-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-Treslin
Plasmid#211404Purposemammalian expression of human Treslin N-terminally fused to mCeruleanDepositorInsertTreslin (C15orf42 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-Mcm2
Plasmid#211395Purposemammalian expression of human Mcm2 N-terminally fused to mCeruleanDepositorInsertMcm2 (MCM2 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAM15
Plasmid#86472PurposeExpresses p12 and p9-mCeruleanDepositorInsertp12, p9-mCerulean
UseSynthetic BiologyTagsmCeruleanExpressionBacterialMutationPromoterpBADAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCer-RPA32
Plasmid#211385Purposemammalian expression of human RPA32 N-terminally fused to mCeruleanDepositorInsertRPA32 (RPA2 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only