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Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
Plasmid#74309PurposeTricistronic expression of TRPV4, ferritin, and mCherry in mammalian cellsDepositorInsertTRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLCKO
Plasmid#73311PurposeLentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynaptoTAG2
Plasmid#175275PurposeAAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.DepositorInsertEGFP-Synaptobrevin-2; tdTomato (Vamp2 )
UseAAVTagsEGFP and tdTomato (P2A cleavage)ExpressionMutationPromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nrp1-Fc-His
Plasmid#72097PurposeExpresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp1 (Nrp1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-EGFP-Kir4.1
Plasmid#52874PurposeExpress EGFP-Kir4.1 fusion protein in astrocytesDepositorInsertKir4.1 (Kcnj10 Rat)
UseAAV; Adeno-associated virus ()TagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Flag-PGC1a-6His
Plasmid#67637PurposeAAV vector expressing PGC1a geneDepositorInsertPGC1a (Ppargc1a Mouse)
UseAAVTags6XHis and Flag tagExpressionMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
Plasmid#78251PurposeCan be used to subclone in tagged gene of interest into inducible motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron inducible expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.1-Fc-His
Plasmid#72098PurposeExpresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp2.1 (Nrp2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3E
Plasmid#50858Purposeexpresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3E (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3E psuedo-phosporylated, non-activatablePromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS mSynCAM1
Plasmid#115496Purposeexpression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expressionDepositorInsertCadm1 (Cadm1 Mouse)
UseTagsExpressionMammalianMutationPromoterchicken beta-actinAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Flpo
Plasmid#98040PurposeSecond-generation rabies viral vector genomeDepositorInsertFlpo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Cre
Plasmid#98039PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-actin prom-dsRed-scramble shRNA
Plasmid#67879PurposeAAV vector to express dsRed sh RNA (scramble)DepositorInsertactin prom-dsRed-scramble shRNA
UseAAV and RNAiTagsExpressionMammalianMutationPromoterchicken beta actinAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE
Plasmid#127865PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationdeleted the c-terminal 20 amino acidsPromoterhSynAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE
Plasmid#127866PurposepAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoterDepositorInsertNos1ap (Nos1ap Mouse)
UseAAVTags3xFLAG and mCherryExpressionMutationC-terminal 20 amino acids onlyPromoterhSynAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP307-pAAV-EFS(No AgeI)-MCS3-pA
Plasmid#113684PurposeEFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.DepositorInsertN/A
UseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArC Long WT
Plasmid#59809PurposeAllows the integration of Myc ArC Long in the genome and Tet-inducible expression.DepositorInsertAurora C (AURKC Human)
UseTagsMycExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(K211TAG)
Plasmid#182660PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK211TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KCC2
Plasmid#104075Purposeexpresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2DepositorInsertKCC2 (b isoform) (Slc12a5 Rat)
UseTagsEGFPExpressionMammalianMutationEcoR1 site in rat KCC2 was removed by silent muta…PromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only