Skip to main content
Addgene
Showing: 5761 - 5780 of 6570 results

  3. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid
    #180017
    Purpose
    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.
    Depositor
    Dirk Hockemeyer
    Article
    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755
    Insert
    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180017-plasmid-map-sequence-id-354058

    Enlarge

  6. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid
    #181975
    Purpose
    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-BBz CAR; tNGFR (NGFR Synthetic, Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NGFR truncation: aa 11-276
    Promoter
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181975-plasmid-map-sequence-id-377682

    Enlarge

  9. pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

    Plasmid
    #122985
    Purpose
    AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT
    Depositor
    Matthew Kennedy
    Article
    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)
    Insert
    mCh-IRES-SspB(milli)-BoNT/B(147-441)
    Use
    AAV
    Tags
    mCh
    Expression
    Mammalian
    Mutation
    SspB contains A58V,R73Q "milli" mutatio…
    Promoter
    human synapsin
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    122985-plasmid-map-sequence-id-238429

    Enlarge

  11. pDONR223_RBM15B_WT_V5

    Plasmid
    #83010
    Purpose
    Gateway Donor vector containing RBM15B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, Jesse Boehm, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    RBM15B (RBM15B Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    R593K; p.1_327deleted
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    83010-plasmid-map-sequence-id-162390

    Enlarge

  13. AICSDP-9:DSP-mEGFP

    Plasmid
    #87424
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DSP
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    DSP Homology Arms with linker-mEGFP (DSP Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87424-plasmid-map-sequence-id-173979

    Enlarge

  16. pDONR223_PIH1D1_WT_V5

    Plasmid
    #82943
    Purpose
    Gateway Donor vector containing PIH1D1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, Jesse Boehm, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    PIH1D1 (PIH1D1 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    M9L, G10E, V224I, P287L
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82943-plasmid-map-sequence-id-162517

    Enlarge

  17. pCIBN-hTRF1-tagRFP-T

    Plasmid
    #103811
    Purpose
    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Mustard Weed, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103811-plasmid-map-sequence-id-199270

    Enlarge

Showing: 5761 - 5780 of 6570 results