Addgene

We narrowed to 16,077 results for: myc tag

Showing: 5701 - 5720 of 16077 results

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pcDNA5 FRT/TO SF-HERC2 (ShB-R)

Plasmid

#55613

Purpose

Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (WT) (HERC2 Human)

Use

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Silent mutations have been incorporated into the …

Promoter

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CXCR4

Plasmid

#98947

Purpose

Mammalian expression plasmid for N-terminal FLAG-tagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Use

Tags

FLAG (dykdddd) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-8his

Plasmid

#149268

Purpose

Mammalian expression plasmid for his-tagged sACE2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA8-Flag-LARS(1-720aa)

Plasmid

#139687

Purpose

Expresses N-teminal Flag tagged aa 1- 720 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA8-Flag-LARS(1-976aa)

Plasmid

#139689

Purpose

Expresses N-teminal Flag tagged aa 1-976 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-FLAG-LEM

Plasmid

#101778

Purpose

Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

MAN1 (LEMD3 Human)

Use

Tags

His14-MBP-SUMO-FLAG

Expression

Bacterial

Mutation

amino acids 6-51 of human MAN1 (LEM domain)

Promoter

T5

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mScarlet-Puro

Plasmid

#169220

Purpose

Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mScarlet-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Puro

Plasmid

#169224

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Hygro

Plasmid

#169225

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Hygro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen-Puro

Plasmid

#169228

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen-P2A-Puro

Use

Targeting vector backbone

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-super TEV protease

Plasmid

#171782

Purpose

Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

MBP-sTEV

Use

Tags

Arg6 tag and Internal His6 tag

Expression

Bacterial

Mutation

No internal TEV cleavage site between the MBP and…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBig1a zz TEV YBBR TPP1 MBP TEV TIN2

Plasmid

#185446

Purpose

Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TPP1 (TPP1 Human)
TIN2 (TINF2 Human)

Use

Tags

MBP, YBBR, and ZZ

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pKGmDUET-NgBR/hCIT

Plasmid

#203173

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Inserts

NUS1 (NUS1 Human)
DHDDS (DHDDS Human)

Use

Tags

Strep-Tag II-TEV and internally 6HIS tagged

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-SAVED-CHAT

Plasmid

#214030

Purpose

Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-dSAVED-CHAT

Plasmid

#214031

Purpose

Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

H375A, C422A

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCaspase

Plasmid

#214034

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pSD025A

Plasmid

#218128

Purpose

Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of gIII-neg

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
M13 pIII-neg

Use

Tags

scP22

Expression

Mutation

W384A, Y386A

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-SRM (sortase recognition motif)-hphMX4

Plasmid

#54488

Purpose

PCR based C-terminal SRM tagging in yeast

Depositor

Eri Sakata

Insert

SRM (sortase-recognition motif)

Use

Yeast genome targeting

Tags

hphMX4

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
AiP1205-Ai227 (TICF-nls-EGFP-ICF-tdTomato-WPRE)

Plasmid

#181761

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

nls-tagged EGFP and tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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