pKGmDUET-NgBR/hCIT
(Plasmid #203173)

• Purpose: Expression of human cis-prenyltransferase complex for purification
• Depositing Lab: William Sessa
• Publication: Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-17361. doi: 10.1074/jbc.M117.806034. Epub 2017 Aug 23.

Backbone

• Vector backbone: pCEP4
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NUS1
• Alt name: NgBR
• Species: H. sapiens (human)
• Entrez Gene: NUS1 (a.k.a. C6orf68, CDG1AA, MGC:7199, MRD55, NgBR, TANGO14)
• Promoter: CMV
• Tag / Fusion Protein:
  • internally 6HIS tagged (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning

Gene/Insert 2

• Gene/Insert name: DHDDS
• Alt name: hCIT
• Species: H. sapiens (human)
• Entrez Gene: DHDDS (a.k.a. CIT, CPT, DEDSM, DS, HDS, RP59, hCIT)
• Tag / Fusion Protein:
  • Strep-Tag II-TEV (N terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry