Addgene

We narrowed to 15,233 results for: LEA

Showing: 5701 - 5720 of 15233 results

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pETNKI-his-3C-LIC-Amp

Plasmid

#108704

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

His-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-Amp

Plasmid

#108707

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Cwc25-pRSETA

Plasmid

#61043

Purpose

Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Cwc25

Use

Tags

TEV site, Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
hJAM-A deltaCT pcDNA3.1

Plasmid

#70074

Purpose

Expresses human junctional adhesion molecule-A amino acids 1-260 in mammalian cells

Depositor

Terence Dermody

Article

Danthi et al J Virol. 2006 Feb;80(3):1261-70.

Insert

junctional adhesion molecule-A (F11R Human)

Use

Tags

Expression

Mammalian

Mutation

deleted C-terminal cytoplasmic tail (amino acids …

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1DCH2AB

Plasmid

#13472

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2AB (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2AB; contains aa 137-367

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
NG0911 pCI-TPI-SMG5(short)-4H

Plasmid

#65805

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Use

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1DCH2A

Plasmid

#13470

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2A; contains aa 47-367

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1DCH2B

Plasmid

#13471

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1DCH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of MKP-1 CH2B; Delta 47-137

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
HOXB4-FLAG-WG-SP65

Plasmid

#21087

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

Insert

HOXB4 (HOXB4 Human)

Use

In vitro t/t

Tags

Expression

Mutation

W to G mutation in YPWM Pbx1 interaction motif, p…

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196b-PGK-GFP-Blast

Plasmid

#31513

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196b (Mir196b Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-324-PGK-GFP-Blas

Plasmid

#31517

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-324 (Mir324 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196a-2-PGK-GFP-Blast

Plasmid

#31511

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196a2 (Mir196a-2 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196bSM-PGK-GFP-Blast

Plasmid

#31514

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196b (Mir196b Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A

Plasmid

#13476

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L17A

Plasmid

#13477

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L17A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 17 to Alanine

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pET28a-ImpBeta1-mEosEM

Plasmid

#218933

Purpose

Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

Importin Beta (KPNB1 Human)

Use

Tags

6xHis and mEos4b (EosEM)

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Expression

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbATML1-1pro

Plasmid

#231149

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1pro

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbATML1-1pro

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-1_NbSTM-5'UTR

Plasmid

#231148

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTR

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbSTM-5?UTR

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

Plasmid

#229840

Purpose

A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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