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Plasmid#88914PurposeFor expression of modified SpyCas9 in e.coli.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only
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EGFP-C3-beta-spectrin
Plasmid#202685Purposefluorescent protein tagged beta-spectrinDepositorInsertEGFP:SPTB(1-2364) (SPTB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-tdTomatof
Plasmid#104060PurposeAlso known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracingDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)
Plasmid#55220DepositorTypeEmpty backboneUseTagsHis6 and MBP, Asn10ExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human TBC1D7 (19-293)
Plasmid#132617Purposeexpresses human TBC1D7 (19-293) in E. coliDepositorInsertTBC1D7 (19-293)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-eNpHR 3.0-EYFP
Plasmid#26775Purpose3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibitionDepositorInserteNpHR 3.0
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP-TARG1
Plasmid#172586PurposeFor transient expression of N-terminal GFP-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot7
Plasmid#37325DepositorInsertHsNot7 (CNOT7 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL2621 (pCas8_Vch)
Plasmid#200893PurposeExpresses human codon-optimized V. cholerae Cas8 from a CMV promoter.DepositorInsertVchCas8
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2623 (pCas6_Vch)
Plasmid#200895PurposeExpresses human codon-optimized V. cholerae Cas6 from a CMV promoter.DepositorInsertVchCas6
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT2dR3-proB
Plasmid#122595PurposeBE PACE selection, express T7 RNA polymerase with degron-GTCCA targetDepositorInsertT7 RNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP341 pHAGE2 CMV TagBFP-3xFLAG
Plasmid#199391PurposeLentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cellsDepositorInsertTagBFP-3xFLAG
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP924 pHAGE2 CMV GFP
Plasmid#199392PurposeLentiviral transfer plasmid for constitutive expression of a GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2622 (pCas7_Vch)
Plasmid#200894PurposeExpresses human codon-optimized V. cholerae Cas7 from a CMV promoter.DepositorInsertVchCas7
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Klentaq
Plasmid#157862PurposePlasmid for the aTc-inducible expression of the KlentaqDepositorInsertKlentaq
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HP138-neo
Plasmid#134247PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selectionDepositorInsertContains Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP
Plasmid#199356PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only