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pPN018

Plasmid

#91664

Purpose

Express sgRNA targeting human SCN2A

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SCN2A (SCN2A Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN018

Plasmid

#91664

Purpose

Express sgRNA targeting human SCN2A

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SCN2A (SCN2A Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN017

Plasmid

#91663

Purpose

Express sgRNA targeting human SCN2A

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SCN2A (SCN2A Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN017

Plasmid

#91663

Purpose

Express sgRNA targeting human SCN2A

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SCN2A (SCN2A Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Rab35b cds in pGem-T Easy

Plasmid

#80556

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab35b (rab35b Zebrafish)

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Rab35b cds in pGem-T Easy

Plasmid

#80556

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab35b (rab35b Zebrafish)

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETFB_WT_V5

Plasmid

#82987

Purpose

Gateway Donor vector containing ETFB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ETFB (ETFB Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETFB_WT_V5

Plasmid

#82987

Purpose

Gateway Donor vector containing ETFB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ETFB (ETFB Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHD2_p.P474R

Plasmid

#81474

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

P474R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHD2_p.P474R

Plasmid

#81474

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

P474R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Rab31 cds in pGem-T Easy

Plasmid

#80507

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab31 (rab31 Zebrafish)

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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Rab31 cds in pGem-T Easy

Plasmid

#80507

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Publication

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab31 (rab31 Zebrafish)

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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IDP254

Plasmid

#87004

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Publication

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postCLAP

Use

Synthetic Biology

Tags

spGFP (1-10)

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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IDP254

Plasmid

#87004

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Publication

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

postCLAP

Use

Synthetic Biology

Tags

spGFP (1-10)

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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PHKA2 gRNA (BRDN0001146244)

Plasmid

#77842

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKA2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKA2 (PHKA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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PHKA2 gRNA (BRDN0001146244)

Plasmid

#77842

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKA2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKA2 (PHKA2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge