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pT7C-DNAJB4

Plasmid

#177656

Purpose

Recombinant protein synthesis of human DNAJB4

Depositor

Jeff Kuret

Publication

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB4 (DNAJB4 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pT7C-DNAJB4

Plasmid

#177656

Purpose

Recombinant protein synthesis of human DNAJB4

Depositor

Jeff Kuret

Publication

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB4 (DNAJB4 Human)

Tags

6xHis-Thrombin

Expression

Bacterial

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY3195

Plasmid

#172695

Purpose

Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Publication

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Punc-25

Tags

GFP 1-10

Expression

Worm

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY3195

Plasmid

#172695

Purpose

Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Publication

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Punc-25

Tags

GFP 1-10

Expression

Worm

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC9A4_STOP

Plasmid

#161412

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A4 (SLC9A4 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC9A4_STOP

Plasmid

#161412

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC9A4 (SLC9A4 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC35D2_STOP

Plasmid

#161288

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D2 (SLC35D2 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pDONR221-SLC35D2_STOP

Plasmid

#161288

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35D2 (SLC35D2 Human)

Expression

Mammalian

Available since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pSICO-CPSF6-4Glu-iRFP670

Plasmid

#167588

Purpose

Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

Depositor

Zandrea Ambrose

Publication

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-4Glu-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Mutation

K547E, R549E, R559E, R561E

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-4Glu-iRFP670

Plasmid

#167588

Purpose

Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

Depositor

Zandrea Ambrose

Publication

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-4Glu-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Mutation

K547E, R549E, R559E, R561E

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Publication

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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ADAP1

Plasmid

#163905

Purpose

Full length mouse ADAP1 for purification from insect cells

Depositor

Martin Loose

Publication

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

Cleavable 6 x HIS tag

Expression

Insect

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

Plasmid

#159911

Purpose

Mutagenesis of Kcnq3

Depositor

Larry Zweifel

Insert

Kcnq3 (Kcnq3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

Plasmid

#159911

Purpose

Mutagenesis of Kcnq3

Depositor

Larry Zweifel

Insert

Kcnq3 (Kcnq3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-HaAEP1

Plasmid

#159530

Purpose

Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Publication

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-HaAEP1

Plasmid

#159530

Purpose

Expresses sunflower (Helianthus annuus) asparaginyl endopeptidase 1 in E. coli

Depositor

Joshua Mylne

Publication

Bernath-Levin et al Chem Biol. 2015 May 21;22(5):571-82. doi

Insert

Helianthus annuus asparaginyl endopeptidase 1 (LOC110922574 Synthetic)

Tags

Six-Histidine tag

Expression

Bacterial

Promoter

T5

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-SCG10/Stathmin-2 [L5/1R]

Plasmid

#140065

Purpose

Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-SCG10/Stathmin-2 [L5/1R]

Plasmid

#140065

Purpose

Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

Depositor

James Trimmer

Publication

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-TLK2-V5_IDG-K

Plasmid

#135251

Purpose

Gateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TLK2-V5 (TLK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-TLK2-V5_IDG-K

Plasmid

#135251

Purpose

Gateway destination clone of TLK2 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TLK2-V5 (TLK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Promoter

Ubiquitin

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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