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phNFL-N98S

Plasmid

#132592

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV2-SMAC-HA-eGFP

Plasmid

#67488

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCDF_AtRaf1/Raf2/BSD2

Plasmid

#229511

Purpose

Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Bsd-CMV>hCDK11A

Plasmid

#239212

Purpose

Expresses CDK11A in mammalian cell lines

Depositor

Jason Sheltzer

Article

Julian et al bioRxiv 2025.08.03.668359; doi: https://

Insert

cyclin dependent kinase 11A (CDK11A Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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PIK3CG gRNA (BRDN0001149186)

Plasmid

#76334

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CG (PIK3CG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIK3CG gRNA (BRDN0001149132)

Plasmid

#76335

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CG

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CG (PIK3CG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX SH2-B-SH2 RA

Plasmid

#46480

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B RK (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639; R555A mutation in t…

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

Plasmid

#136659

Purpose

Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD9:TH1477 chimera
sgRNA for St1Cas9
APOBEC-1 (APOBEC1 Human)
UGI
HA tag

Use

CRISPR and Synthetic Biology

Tags

2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCUX1.1.0-gDNA

Plasmid

#112434

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1

Depositor

Kevin White

Insert

CUX1 (CUX1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-PIK3CA-E545K

Plasmid

#249477

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Article

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E545K

Promoter

EF-1a

Available Since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-7 [N183/15R-2b]

Plasmid

#219485

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody. (Qki Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK11B-V5-miniTurbo_IDG-K

Plasmid

#189914

Purpose

Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK11B (CDK11B Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-FGF13/FHF2 [N91/27.1R]

Plasmid

#114525

Purpose

Mammalian Expression Plasmid of anti-FGF13/FHF2 (Human). Derived from hybridoma N91/27.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-FGF13/FHF2 (Homo sapiens) recombinant mouse monoclonal antibody (FGF13 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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PIK3CA gRNA (BRDN0001146906)

Plasmid

#77417

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CA (PIK3CA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146011)

Plasmid

#77065

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11B gRNA (BRDN0001146017)

Plasmid

#77066

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11B (CDK11B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v1 (B.1.617.1 v1)

Plasmid

#212428

Purpose

Encodes SARS-CoV-2 spike G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCR4_miniFXN_7

Plasmid

#234360

Purpose

Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

Depositor

Marek Napierala

Article

Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

Insert

FXN (FXN Human)

Use

Cloning

Tags

Flag Tag

Mutation

There is a partial deletion in the 5'UTR of …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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