Skip to main content
Addgene
Showing: 5661 - 5680 of 11168 results

  2. pD649-HAsp-IL1RL2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156753
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1RL2 (IL1RL2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156753-plasmid-map-sequence-id-302615

    Enlarge

  3. Myc-Bnip3-T181A

    Plasmid
    #197556
    Purpose
    Mammalian expression of myc-tagged mouse Bnip3 with an alanine substitution of threonine-181. Based on Myc-Bnip3FL (#100796)
    Depositor
    Joseph Gordon
    Article
    Martens et al Cell Death Dis. 2021 Nov 26;12(12):1105.
    Insert
    BCL2 Interacting Protein 3 (Bnip3 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Threonine 181 changed to alanine
    Promoter
    CMV
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    197556-plasmid-map-sequence-id-386257

    Enlarge

  6. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

    Enlarge

  10. pLKO_S.V5.FLAG_NGFR

    Plasmid
    #158337
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.V5.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158337-plasmid-map-sequence-id-335194

    Enlarge

  11. pLKO_S.V5.NWS_NGFR

    Plasmid
    #158338
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.V5.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158338-plasmid-map-sequence-id-335195

    Enlarge

  12. pLKO_S.V5.AU1_NGFR

    Plasmid
    #158340
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.V5.AU1
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158340-plasmid-map-sequence-id-330654

    Enlarge

  13. pLKO_S.V5.VSVg_NGFR

    Plasmid
    #158341
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.V5.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158341-plasmid-map-sequence-id-330657

    Enlarge

  14. pLKO_Ollas.V5.FLAG_NGFR

    Plasmid
    #158316
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.V5.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158316-plasmid-map-sequence-id-335191

    Enlarge

  15. pLKO_Ollas.V5.NWS_NGFR

    Plasmid
    #158317
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.V5.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158317-plasmid-map-sequence-id-329922

    Enlarge

  16. pLKO_Ollas.V5.HA_NGFR

    Plasmid
    #158318
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.V5.HA
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158318-plasmid-map-sequence-id-329923

    Enlarge

  17. pLKO_Ollas.AU1.V5_NGFR

    Plasmid
    #158319
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.AU1.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158319-plasmid-map-sequence-id-329924

    Enlarge

  18. pLKO_Ollas.V5.VSVg_NGFR

    Plasmid
    #158320
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.V5.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158320-plasmid-map-sequence-id-329925

    Enlarge

  19. pLKO_AU1.Ollas.FLAG_NGFR

    Plasmid
    #158323
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    AU1.Ollas.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158323-plasmid-map-sequence-id-330144

    Enlarge

  20. pLKO_AU1.Ollas.NWS_NGFR

    Plasmid
    #158326
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    AU1.Ollas.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158326-plasmid-map-sequence-id-330446

    Enlarge

Showing: 5661 - 5680 of 11168 results