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Plasmid#112265PurposeITRACKbeta/IDOCKSbeta Donor constructDepositorInsertprotein kinase c beta (Prkcb Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS/ES-EnvA-VSVg-WPRE-pA
Plasmid#87221Purposeto package wild type EnvA psudotyped lentivirusDepositorInsertEnvA-VSVg
UseMouse Targeting and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
mcherry-Myo 9b
Plasmid#134918PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.DepositorInsertMyo 9b (rat) with 3' UTR (Myo9b Rat)
UseTagsmcherryExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUltra-U6-gRNAs1-5
Plasmid#178569PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editingDepositorInsert5 gRNA-tRNA array with U6 promoter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-Sp dCas9-mVenusN
Plasmid#191396PurposeSpdCas9 fused to N-terminal part of mVenusDepositorInsertN-terminal part of split mVenus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s-CAAX
Plasmid#52228PurposePlasma membrane targeted GCaMP6s, calcium sensorDepositorInsertGCaMP6s-CAAX
UseTags6xHis and CAAXExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-CaMKIID
Plasmid#186827PurposeExpresses FLAG-CaMKIID in mammalian cellsDepositorInsertCamk2d (Camk2d Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1-14-mVenusC
Plasmid#191393PurposeMBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptSKII+ U6-sgRNA(F+E) empty
Plasmid#74707PurposeEncodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequenceDepositorInsertU6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ADP-MCS-FLAG
Plasmid#192360PurposeAdipocyte-specific overexpression of genesDepositorTypeEmpty backboneUseAAVTags3xflagExpressionMammalianMutationPromoteradiponectin promoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+24)
Plasmid#199163Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+24)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
neurod1:CaMPARI
Plasmid#173479PurposeCaMPARI is driven by -5kb neurod1 promoter.DepositorInsertCaMPARI
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HDAC6-FLAG
Plasmid#30482DepositorInsertHDAC6 (HDAC6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP
Plasmid#99731Purposeoverexpression of PDGFRa and CopGFPDepositorInsertPdgfra (Pdgfra Mouse)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRG705_lexO-AsiSI_LexA-TF_LEU2MX
Plasmid#154817PurposeInducible expression of AsiSI endonuclease in S. cerevisiaeDepositorInsertsAsiSI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 1-1290
Plasmid#78579PurposeExpression flag-USP19 1-1290 in mammalian cellsDepositorInsertflag-USP19 1-1290 (USP19 Human)
UseTagsflagExpressionMammalianMutationmutate 1291 residue to stop condonPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Nrf2
Plasmid#67636PurposeAAV vector expressing Nrf2 geneDepositorInsertNrf2 (Nfe2l2 Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CEPT1
Plasmid#119079PurposeVisualize localization of ER enzyme CEPT1, which converts diacylglycerol precursors to phosphatidylethanolamine or phosphatidylcholineDepositorInsertCEPT1 (CEPT1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only