Addgene

We narrowed to 16,077 results for: myc tag

Showing: 5641 - 5660 of 16077 results

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CAGGS-SAFB-NLS-3XFLAG-V5

Plasmid

#196089

Purpose

Expresses mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Mutation

Promoter

CAGGS

Availability

Academic Institutions and Nonprofits only

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pET28a(ICOS)

Plasmid

#177903

Purpose

Bacterial expression of his10-tagged human ICOS Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

ICOS (aa 162-199 only) (ICOS Human)

Use

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pETM33_AP2B1_adaptin

Plasmid

#178467

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

AP2B1_adaptin (AP2B1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_CLTC_Clathrin propeller

Plasmid

#178471

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLTC_Clathrin propeller (CLTC Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM33_DCUN1D1_PONY

Plasmid

#178473

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

DCUN1D1_PONY (DCUN1D1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNB1_HEAT

Plasmid

#178494

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNB1_HEAT (KPNB1 Human, Synthetic)

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

T7/LacO

Availability

Academic Institutions and Nonprofits only

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G12-CASE

Plasmid

#190714

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.

Inserts

GNB3
GNGT9
GNA12

Use

Luciferase

Tags

Nluc is inserted at A133/F134 within GNA12 and cp…

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMV_AA091

Plasmid

#216106

Purpose

CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression with modified trRNA to recruit activators via PP7 tags

Use

CRISPR and Lentiviral; Assembled vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-N FLAG

Plasmid

#12541

Depositor

Titia de Lange

Insert

N FLAG

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pENTR-R2L3-cGR-ID

Plasmid

#139541

Purpose

Provides GR protein tag N-terminal to IRES-HisD-pA cassette on Gateway entry vector

Depositor

Minoru Ko

Insert

GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.BRWD3-V5H

Plasmid

#48624

Purpose

Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells

Depositor

Aziz Sancar

Article

Ozturk et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

BRWD3 (BRWD3 Fly)

Use

Tags

V5 and His tags

Expression

Mammalian

Mutation

no mutations, stop was removed to fuse V5His at C…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pYFP-ErbB2ΔC990

Plasmid

#66946

Purpose

Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Use

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

Deletion of C990. (see depositor comment below on…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pYFP-ErbB2ΔC776

Plasmid

#66947

Purpose

Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Use

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

Deletion of C776 (see depositor comment below on …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pLV-CDH5-mCitrine-IRES-puro

Plasmid

#85143

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Expression

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A Human, (*see below))
humanized birA

Use

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Use

Tags

sfCherry11x4

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Use

Tags

sfCherry11

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/DEST/hCx43-E2HA-stop

Plasmid

#40909

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 internal HA tag (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

HA tag inserted in second extracellular loop

Promoter

CMV

Availability

Academic Institutions and Nonprofits only