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  4. pYG215

    Plasmid
    #200837
    Purpose
    Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    Promoter
    P-LlacO-1
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200837-plasmid-map-sequence-id-405511

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  8. AV13_pCAG.Cas9.gRNA.NT

    Plasmid
    #199260
    Purpose
    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Inserts
    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199260-plasmid-map-sequence-id-391374

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Showing: 5641 - 5660 of 14729 results