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Plasmid#221702PurposeLentiviral expression of human RNF8 (R42A) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationR42APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-C403S
Plasmid#221703PurposeLentiviral expression of human RNF8 (C403S) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationC403SPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-S157A
Plasmid#221704PurposeLentiviral expression of human RNF8 (S157A) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationS157APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4.1-Myc-RNF8
Plasmid#221676PurposeMammalian expression of Myc-tagged human RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD090A
Plasmid#218132PurposeHybrid circuit displaying mmCRBN bait and driving expression of luxABDepositorInsertsUseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD138A
Plasmid#218130PurposeHybrid circuit displaying hsCRBN bait (P22 anchor) and driving expression of luxABDepositorInsertsUseTagsscP22ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD094B
Plasmid#218131PurposeHybrid circuit displaying mmCRBN bait and driving expression of gIIIDepositorInsertsUseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM185A
Plasmid#218126PurposeHybrid circuit displaying hsCRBN bait and driving expression of luxABDepositorInsertsUseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 WT
Plasmid#212946PurposeVector used for expression of AtSLAC1 WT in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 6D
Plasmid#212947PurposeVector used for expression of AtSLAC1 6D in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
UseTagsExpressionMammalianMutationT62D/S65D/S107D/S124D/S146D/S152DPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF148
Plasmid#155330PurposeBinary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette.DepositorInsertLB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc253
Plasmid#207464PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253DepositorInsertMTdTc253 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationSer253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc188
Plasmid#207463PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188DepositorInsertTdTc188 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys216Ser, Cys302Ala, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc180
Plasmid#207462PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180DepositorInsertTdTc180 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationGlu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTc302
Plasmid#207461PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertTdTc302 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-HAT
Plasmid#202557PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsHistidine-Affinity-tag (HAT) and TEV cleavable si…ExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS2-TEV-Twin-Strep
Plasmid#202529PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cellDepositorInsertSGMS2 (SGMS2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TGA)PromoterCAG and chicken β-actin promoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFDPS #2
Plasmid#198760Purposeconditional knockdown of FDPSDepositorInsertshFDPS #2 (FDPS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFDPS #1
Plasmid#198759Purposeconditional knockdown of FDPSDepositorInsertshFDPS #1 (FDPS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D
Plasmid#198765Purposeexpression of hOCT4 mutantsDepositorInserthOCT4 236D (POU5F1 Human)
UseTagsFLAGExpressionMammalianMutationS236DPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only