Addgene

We narrowed to 16,086 results for: LEA

Showing: 5621 - 5640 of 16086 results

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pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

Plasmid

#87694

Purpose

Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

ERT2-PhoCl-Cre-PhoCl-ERT2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNES-EGFP-C1-flex-PABD-Spo20

Plasmid

#220081

Purpose

Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

Spo20 (SPO20 Budding Yeast)

Use

Tags

X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

Expression

Mammalian

Mutation

amino acids 51-91

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pROSA26-promoter

Plasmid

#21710

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 promoter (Gt(ROSA)26Sor Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pY011 (pcDNA3.1-hLb2Cpf1)

Plasmid

#69983

Purpose

Expresses humanized Lb2Cpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLb2Cpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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CANE-LV envelope

Plasmid

#86666

Purpose

pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

Depositor

Fan Wang

Article

Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

Insert

CANE-LV envelope

Use

Lentiviral, Mouse Targeting, and Synthetic Biolog…

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only
pRmHA3-CH2F2-CG9866

Plasmid

#52286

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Use

Tags

2xHA 2xFLAG

Expression

Insect

Mutation

Promoter

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-D53A-GFP

Plasmid

#208778

Purpose

MSCV vector expressing D53A mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

Changed aspartic acid 53 to alanine (D53A)

Promoter

Available since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTU424

Plasmid

#178983

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDN80

Plasmid

#61349

Purpose

LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Type

Empty backbone

Use

Tags

AsLOV2 wt and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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3xflag-APEX2-NES

Plasmid

#164623

Purpose

Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

NES

Use

Tags

3xFLAG and APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO1

Plasmid

#24316

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO1 (AGO1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2010

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1.ARRDC1

Plasmid

#38320

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR

Plasmid

#140051

Purpose

Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-spCas9-N57

Plasmid

#169920

Purpose

Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

SpCas9-N57

Use

CRISPR

Tags

Expression

Mutation

N57 is the PAI domain of SB100X

Promoter

CAG

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSin-GFP-Fg

Plasmid

#174307

Purpose

Lentiviral expression of GFP

Depositor

Bruce Beutler

Article

Yue et al Science. 2021 May 14;372(6543). pii: 372

Insert

GFP (eGFP Aequorea victoria)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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