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Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CrkII
Plasmid#50731Purposemammalian expression of CrkIIDepositorInsertCrkII (CRK Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_300/pcDNA3
Plasmid#51983Purposemammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nMDepositorInsertNano-lantern_CaM_E104Q-3GS
UseTagsExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Arl13b1
Plasmid#40870DepositorInsertArl13b (Arl13b Mouse)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
CaYin1 NES
Plasmid#33318DepositorInsertCaYin1 NES
UseTagsNES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I/I997A
Plasmid#111829PurposeVinculin tension sensor with point mutations affecting ability to bind talin and actinDepositorInsertVinculinTS (I997A)
UseTagsExpressionMammalianMutationchanged vinculin alanine 50 to isoleucine and iso…PromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Wnt9B
Plasmid#35919DepositorInsertWnt9B (WNT9B Human)
UseTagsExpressionMammalianMutationSNP Met106-->ThrPromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
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IO001: pMVP (R4-R3) eGFP
Plasmid#121730PurposepMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA179
Plasmid#128537PurposeExpresses anti-Prunus dulcis scFv AbDepositorInsertscFv Ab clone PD1F6
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-T7-hAIM2
Plasmid#51541PurposeExpress T7 and GFP-tagged hAIM2 in mammalian cellsDepositorInsertAIM2 (AIM2 Human)
UseTagsGFP and T7ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
TN-XXL pRSETB
Plasmid#45796PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
UseTags6xHIS, ECFP, and YFPExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpyTag-GlpG
Plasmid#79054PurposeExpresses SpyTagged GlpG proteinDepositorInsertSpyTag-GlpG (glpG )
UseTags6xHis and SpyTagExpressionBacterialMutationGlpG protein employed here corresponds to residue…PromoterTrcAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP GFP
Plasmid#45237PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD, INCENP-Δcen, GFP
UseTagsDNA binding domain of CENP-B and GFPExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)PromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-Wnt11-V5
Plasmid#35941DepositorInsertWnt11 (wnt11f2 Human)
UseTagsV5ExpressionMammalianMutationNonePromoterCMVAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1_160-236-MCS
Plasmid#60546PurposeCloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALMDepositorInsertPAmCherry1 C-terminal fragment
UseCloningTagsExpressionMutationamino acids 160-236PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1_1-159-MCS
Plasmid#60545PurposeCloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALMDepositorInsertPAmCherry1 N-terminal fragment
UseCloningTagsExpressionMutationamino acids 1-159PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVC
Plasmid#175063Purposeexpresses mVenus and mCherry pair in mammalian cellsDepositorInsertmVenus + mCherry
UseTagsExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-1.10a
Plasmid#69589PurposeOT-II TCR a chain cDNA in pCRIIDepositorInsertTCR alpha clone 1.1 (Tcra Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only