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Plasmid#72209PurposeMammalian expression of aa 1-517 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 518-860PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM4
Plasmid#72211PurposeMammalian expression of aa 373-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 1-372PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2-FDM5
Plasmid#72212PurposeMammalian expression of aa 518-860 of human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationΔ 1-517PromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 LIAS-1
Plasmid#184478PurposeLentivirus gRNA targeting human LIAS geneDepositorInsertLIAS (LIAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 LIAS-2
Plasmid#184479PurposeLentivirus gRNA targeting human LIAS geneDepositorInsertLIAS (LIAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA2
Plasmid#98400PurposeLentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a HsDHFR-bio-10His
Plasmid#193883PurposeExpresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequenceDepositorInserthuman dihydrofolate reductase (DHFR) (DHFR Human)
UseTagsHis tag and biotinylation sequenceExpressionBacterialMutationCodon optimizedPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSDC803_IKZF1_mutant
Plasmid#224113PurposeInsect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blottingDepositorInsertIKZF1 (IKZF1 Human)
UseTagsFlag-TEVExpressionInsectMutationResidues 140-196, Q146A and G151NPromoterpolyhedrinAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgFAAH
Plasmid#209197PurposeMutagenesis of FaahDepositorInsertFaah (Faah Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterCMVAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Downstream_gRNA_2
Plasmid#195137PurposegRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Downstream gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_CRISPRi_gRNA_2
Plasmid#195133PurposegRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherryDepositorInsertMDM4 CRISPRi gRNA 2 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_CRISPRi_gRNA_1
Plasmid#195132PurposegRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherryDepositorInsertMDM4 CRISPRi gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDM4_Deletion_Downstream_gRNA_1
Plasmid#195136PurposegRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletionDepositorInsertMDM4 Deletion Downstream gRNA 1 (MDM4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pITGA5_1745G>U
Plasmid#173812Purposemir218 binding site2 point mutationDepositorInsertNT1745 ITGA5 point mutation (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAmpRAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pITGA5_Q853L
Plasmid#174008PurposeFibronectin receptor binding site point mutationDepositorInsertFibronR ITGA5 point mutation (ITGA5 Human)
UseTagsExpressionMammalianMutationPromoterAmpRAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMVtight-PLXNB2 iOE
Plasmid#176849Purposelentiviral vector for doxycycline-inducible PLXNB2 overexpressionDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationfull length PLXNB2PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-hPLXNB2_PGK_Neo
Plasmid#176848Purposelentiviral vector for constitutive PLXNB2 overexpressionDepositorInserthPLXNB2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationfull length PLXNB2PromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
EIF2B3-C424G
Plasmid#172091PurposeIn vitro transcription for rescue experimentsDepositorInsertEIF2B3 (EIF2B3 Human)
UseTagsExpressionMammalianMutationc.1270T>GPromoterCMVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EIF2B3-V398fs
Plasmid#172090PurposeIn vitro transcription for rescue experimentsDepositorInsertEIF2B3 (EIF2B3 Human)
UseTagsExpressionMammalianMutationc.1193-1194delTGPromoterCMVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EIF2B3-S369fs
Plasmid#172089PurposeIn vitro transcription for rescue experimentsDepositorInsertEIF2B3 (EIF2B3 Human)
UseTagsExpressionMammalianMutationc.1106_1113delPromoterCMVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only