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Plasmid#105571PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNADepositorInsertMAP4 (Map4 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G'
Plasmid#209124PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'DepositorInsertSUMO-A'-l4-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Vimentin - APEX2 in pECFP
Plasmid#66170PurposeAPEX2 fused to the C-terminus human vimentinDepositorInserthuman Vimentin - APEX2 (VIM Synthetic, Human)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmiHiFiCas9
Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB_Cdc42-TomKat-sensor-CAAX
Plasmid#172473PurposePiggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.DepositorInserttdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
UsePiggybac transposonTagspolybasic and CAAX motif from Kras, tdKatushka2, …ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK198 (GAL-OsTIR1)
Plasmid#140655PurposeGAL-OsTIR1(WT)DepositorInsertOsTIR1(WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-metap2
Plasmid#100510PurposeExpresses FLAG tagged metap2 in mammalian cells to make lentivirusDepositorInsertMETAP2 (METAP2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hOct4
Plasmid#20072DepositorInserthOct4 (POU5F1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Short P2A
Plasmid#164118PurposeDetect the short Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-MXT GIRK2
Plasmid#20675DepositorInsertMus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
UseXenopus expressionTags3' UTR Xenopus B- globin and 5' UTR Xen…ExpressionMutationA369VPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SPI1_P2A_Hygro_Barcode
Plasmid#120482PurposeBarcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER- Po Long P2A
Plasmid#164117PurposeDetect the long Peroxisomes-mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP and YFP Mt-ER- Po Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS
Plasmid#107319PurposeExpresses SpCas9 fused to dSaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to nuclease dead SaCas9 (D10A, N5…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-iLIDx6
Plasmid#103779Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertmCherry-iLIDx6
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
nLuc-PTHR1
Plasmid#162577PurposeExpresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cellsDepositorInsertParathyroid Hormone Receptor 1 (PTH1R Human)
UseTagsNanolucExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FL tension sensor module
Plasmid#101170PurposeExpresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.DepositorInsertYPet(short)-FL-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-6XPRE-GFP
Plasmid#206171PurposepMVP Gateway destination vector (3rd generation lentiviral vector) containing 6 consensus PGR binding sites upstream of a minimal 2YBTATA promoterDepositorInsert6X PRE-eGFP
UseLentiviralTagsExpressionMutationPromoter2YBTATAAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1082_Rad21_STOP
Plasmid#156450PurposeFor transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codonDepositorInsertCas9, RAD21 (Rad21 Mouse)
UseCRISPRTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only