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Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorInsertMYO7B sgRNA (MYO7B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLhE_AW-pHG165
Plasmid#90062PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE_AW
UseTagsExpressionBacterialMutationI48V/Q55A/Q60RPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhS-pHG165a
Plasmid#90040PurposeConstitutive expression of a chimeric LacI:GalS transcription repressorDepositorInsertLLhS
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhF-pHG165a
Plasmid#90041PurposeConstitutive expression of a chimeric LacI:FruR transcription repressorDepositorInsertLLhF
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhT-pHG165a
Plasmid#90042PurposeConstitutive expression of a chimeric LacI:TreR transcription repressorDepositorInsertLLhT
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhR-pHG165a
Plasmid#90043PurposeConstitutive expression of a chimeric LacI:RbsR transcription repressorDepositorInsertLLhR
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhA-pHG165a
Plasmid#90044PurposeConstitutive expression of a chimeric LacI:AscG transcription repressorDepositorInsertLLhA
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhE-pHG165
Plasmid#90045PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhC-pHG165a
Plasmid#90046PurposeConstitutive expression of a chimeric LacI:CytR transcription repressorDepositorInsertLLhC
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tagger
Plasmid#129396PurposeExpresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoterDepositorInsertrpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
UseTagsFLAG, HA, and V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGH10 - RlucFluc minus 1 HIV-1
Plasmid#198224PurposeFluorescence reporters for -1 ribosomal frameshifting in mammalian cellsDepositorInsertsRenilla luciferase
Firefly luciferase
UseTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE
Plasmid#198337PurposePB plasmid expressing Halo-tagged PP7 coat proteinDepositorInsertPP7 coat protein 3x Halo-GB1
UsePiggybacTagsHaloTagExpressionMammalianMutationPromoterEf1aAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXM1
Plasmid#153136PurposeExpression of Flag tagged human FOXM1 in mammalian cellsDepositorInserthuman FOXM1 (FOXM1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-LD)
Plasmid#214420PurposeDetection of ER-lipid droplet contact siteDepositorInsertRspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(mito-LD)
Plasmid#214421PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
UseLentiviralTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-FAB(ER-mito)
Plasmid#214423PurposeDetection of ER-mito contact siteDepositorInsertRspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
UseLentiviralTagsCFAST10-Halo and RspAN-FRB-V5ExpressionMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Hygro_GCaMP6s
Plasmid#164591PurposeStable integration and expression of GCaMP6s in mammalian cellsDepositorInsertGCaMP6s
UseLentiviralTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RLuc8
Plasmid#87121PurposeMammalian expression vector expressing mutant vesrion of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.DepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationA55T, C124A, S130A, K136R, A143M, M185V, M253L, a…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
FAB(mito-LD)
Plasmid#214425PurposeDetection of mito-lipid droplet contact siteDepositorInsertRspAN-3xHA-V5-mito
UseTagsCFAST-Halo and RspAN-3XHA-V5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-H2B-mClover3
Plasmid#171489PurposeT7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNADepositorInsertH2B (H2BC11 Human)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only