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Plasmid#124663PurposeExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coilDepositorInsertSpyCatcher002-oTet
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oTri
Plasmid#124662PurposeExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coilDepositorInsertSpyCatcher002-oTri
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
UseTagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHex
Plasmid#124670PurposeExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coilDepositorInsertSpyCatcher002-oHex
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oPent
Plasmid#124664PurposeExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coilDepositorInsertSpyCatcher002-oPent
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
UseTagsGSGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oHept
Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003
Plasmid#133566PurposeExpresses EGFP-Talin head-SpyCatcher003 in mammalian cellsDepositorInsertEGFP-Talin head-SpyCatcher003
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV (AAV1)
Viral Prep#140135-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iFlpV (AAV1)
Viral Prep#140137-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-iFlpV (#140137). In addition to the viral particles, you will also receive purified pAAV-EF1a-iFlpV plasmid DNA. EF1a-driven, light-inducible iFlpV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iDreV (AAV1)
Viral Prep#140136-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-iDreV (#140136). In addition to the viral particles, you will also receive purified pAAV-EF1a-iDreV plasmid DNA. EF1a-driven, light-inducible iDreV recombinase for spatiotemporally precise optogenomic modifications. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iCreV (AAV PHP.eB)
Viral Prep#140135-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-iCreV (#140135). In addition to the viral particles, you will also receive purified pAAV-EF1a-iCreV plasmid DNA. EF1a-driven, light-inducible iCreV recombinase for spatiotemporally precise optogenomic modifications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only