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Plasmid#223950PurposeGateway cloning of genes of interest with a C-terminal mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmECFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only
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pRS415 GPDpro-RNQ1-CFP
Plasmid#224887PurposeLow copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsCFPExpressionYeastMutationPromoterGPDAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:mApple-he1.1:CFP
Plasmid#184812PurposeUsed to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic lineDepositorArticleInsertQUAS:mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK ARF6(Q67L)-CFP
Plasmid#200428PurposeMammalian expression of Q67L mutation in ARF6DepositorInsertARF6 (ARF6 Human)
UseTagsCFPExpressionMammalianMutationQ67LPromoterPGKAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Slack Slo2.2 max1-ECFP
Plasmid#197318PurposePlasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunitDepositorInsertKCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)
UseTagsECFPExpressionMammalianMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAvicFP1-T2A-mCherry pdrf1-GW
Plasmid#168073PurposeProduces equimolar expression of mAvicFP1 and mCherry for use in yeastDepositorInsertmAvicFP1-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-FRB1C_no-linker
Plasmid#162434Purposefor use in CIT (chemically induced trimerization) systemDepositorInsertLyn-CFP-FRB1C_no_linker
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-HOXD13(+7A)-IDR
Plasmid#145256PurposeExpresses fusion of ECFP-LacI and HOXD13+7A IDRDepositorInsertHOXD13(+7A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LD101: pMVP (L3-L2) eCFP + WPRE
Plasmid#121775PurposepMVP L3-L2 entry plasmid, contains eCFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest in lentivirus vectors.DepositorInserteCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC701: pMVP (L3-L2) eCFP + polyA
Plasmid#121767PurposepMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.DepositorInserteCFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO601: pMVP (R4-R3) eCFP open
Plasmid#121735PurposepMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KP401: pMVP (L5-L4) eCFP-P2A
Plasmid#121712PurposepMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.DepositorInserteCFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yoeCFP-T2A-mCherry
Plasmid#118445PurposeProduces equimolar expression of yoeCFP and mCherryDepositorInsertyoeCFP-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mCFP
Plasmid#105136PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mCFP
UseTags3nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor CFP-hDF
Plasmid#72590PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGDF8 donor CFP-hDF
Plasmid#72591PurposePlasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158) in pcDNAI/Amp
Plasmid#60895PurposeThis vector attaches CFP(1-158) to the N-terminus of a protein.DepositorInsertCFP(1-158)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-CFP
Plasmid#50482Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsCFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the CFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:ECFP
Plasmid#113880Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagsECFPExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECFP18aaEYFP
Plasmid#109330PurposeECFP-EYFP FRET positive controlDepositorInsertECFP-EYFP FRET positive control
UseTagsExpressionMammalianMutationECFP linked to EYFP with 18 aa linker GLRSRAQASNS…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only