-
Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa-SpCas9D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smV5-4x6T-WPRE
Plasmid#196416PurposeAstrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rabbit GFP Cav 2.2 pMT2
Plasmid#58737Purposeexpression of GFP tagged rabbit Cav2.2 in mammalian cellsDepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYOD1_P2A_Hygro_Barcode
Plasmid#120464PurposeBarcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA pCAGGS
Plasmid#206076Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain IIDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-FNIP1
Plasmid#72293PurposeoverexpressionDepositorInsertFNIP1 (FNIP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_RUNX1_P2A_Hygro_Barcode
Plasmid#120475PurposeBarcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_mVenus
Plasmid#113893PurposeMammalian (inducible) expression of cytosolic mVenusDepositorInsertmonomeric Venus
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAV1-K*R-HA
Plasmid#27765DepositorInsertcaveolin-1, lysine null mutant (CAV1 Human)
UseTagsHAExpressionMammalianMutationAll lysines mutated to arginines (K*R)PromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-2XPRE-GFP
Plasmid#206169PurposepMVP Gateway destination vector (3rd generation lentiviral vector) containing 2 consensus PGR binding sites upstream of a minimal 2YBTATA promoterDepositorInsert2X PRE-eGFP
UseLentiviralTagsExpressionMutationPromoter2YBTATAAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LDB-14-p65
Plasmid#158985PurposeExpresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cellsDepositorInsertLDB-14-p65
UseTagsV5 tag , 6xHis tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL(D320A)-FLAG
Plasmid#111291Purposemammalian expressionDepositorInserthuman CLYBL D320A (CLYBL Human)
UseTagslinker and FLAG tag: TGGGSGGSGGSMDYKDDDDKExpressionMammalianMutationD320APromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Actin-7
Plasmid#57339PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SARS-CoV-2 NSP13
Plasmid#176492PurposeExpresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.DepositorInsertSARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationI570 from SARS-CoV NSP13 was mutated to V to conv…PromoterT7Available sinceJan. 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits