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Plasmid#145265PurposeMammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLSDepositorInsertHOXA13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS
Plasmid#145262PurposeMammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLSDepositorInsertHOXD13(+8A)-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationA-repeat expandedPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXD13-Adel-15A-IDR
Plasmid#145243PurposeExpresses fusion of GAL4 DNA-binding domain and HOXD13-Adel-15A IDRDepositorInsertHOXD13-Adel-15A-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13-44A-IDR
Plasmid#145237PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDRDepositorInsertHOXA13-44A-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HOXA13-15A-IDR
Plasmid#145236PurposeExpresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDRDepositorInsertHOXA13-15A-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-HEY2-Adel-IDR
Plasmid#145234PurposeExpresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDRDepositorInsertHEY2-Adel-IDR (HEY2 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-BHLHE41-Adel-IDR
Plasmid#145228PurposeExpresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDRDepositorInsertBHLHE41-Adel-IDR (BHLHE41 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-mHdac1-L2
Plasmid#122307PurposeExpresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Hdac1 (Hdac1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
289aa ELL2
Plasmid#127266PurposeFor in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.DepositorInsertNH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to Ileu. Contains 289…PromoterT7Available sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2 Met133, 138, 186 to Ileu
Plasmid#127269PurposeFor in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 IleuDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu HA tag after M186PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2 Met133, 138, 186 to Ileu
Plasmid#127271PurposeFor in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th MetDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu with HA tag after 8th MetPromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2 M133, 138, 186ILeu
Plasmid#127276PurposeExpresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt functionDepositorInsertELL2 (ELL2 Human)
UseTagsExpressionMammalianMutationM133, 138, 186 to Ileu, HA tag at XbaI after Met …PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Adeno BN
Plasmid#101823PurposeAdenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1DepositorUseAdenoviralTagsFLAG-Cas9ExpressionMutationPromoterAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
RV-cag-Dio-Kif5c-2A-tdt
Plasmid#87668Purposeretroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560DepositorInsertKif5C-P2A-mTdt (Kif5c Rat)
UseRetroviralTagspalmitylationExpressionMutationmotor domain aa1-560 present, V40A, E125V (see de…PromoterCAGAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-MDM4
Plasmid#195140PurposeTet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassetteDepositorInsertMDM4 (MDM4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Gata6
Plasmid#72694PurposeDoxycyline regulated expression of Gata6 in mammalian cellsDepositorInsertGata 6 (Gata6 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag
Plasmid#216250PurposeHDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlagDepositorInsertHDR template for human SEC61B (SEC61B Synthetic, Human, HDR template)
UseCRISPR; Hdr templateTagsBSD.P2A.miniIAA7.3xFlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HP1a-mCherry
Plasmid#185012PurposeBacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion ProteinDepositorInsertCBX5 (HP1a) (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSRB274_pAC8-eGFP-hsCRBN
Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only