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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,087 results for: grna

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Showing: 5541 - 5560 of 31087 results

  1. pAGK60

    Plasmid
    #228120
    Purpose
    Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228120-plasmid-map-sequence-id-455404

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  2. pAGK55

    Plasmid
    #228114
    Purpose
    Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228114-plasmid-map-sequence-id-455303

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  7. pAGK111

    Plasmid
    #228126
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228126-plasmid-map-sequence-id-455418

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  8. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid
    #114729
    Purpose
    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene
    Depositor
    Martine Roussel
    Publication
    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA M5, sgRNA M7, sgRNA M9
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114729-plasmid-map-sequence-id-222610

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  20. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Publication
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    167547-plasmid-map-sequence-id-332733

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Showing: 5541 - 5560 of 31087 results