Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 16,776 results for: grna

Showing: 5541 - 5560 of 16776 results

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C8orf44-SGK3 gRNA (BRDN0001146567)

Plasmid

#75906

Purpose

3rd generation lentiviral gRNA plasmid targeting human C8orf44-SGK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001145211)

Plasmid

#75885

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001147480)

Plasmid

#75886

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001162403)

Plasmid

#75887

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS2

Plasmid

#69234

Purpose

U6 driven SpCas9 sgRNA expression for TS2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS3

Plasmid

#69235

Purpose

U6 driven SpCas9 sgRNA expression for TS3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TS4

Plasmid

#69236

Purpose

U6 driven SpCas9 sgRNA expression for TS4 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-KANK3

Plasmid

#69238

Purpose

U6 driven SpCas9 sgRNA expression for KANK3 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

KANK3 sgRNA (KANK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-PLXNB2

Plasmid

#69240

Purpose

U6 driven SpCas9 sgRNA expression for PLXNB2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

PLXNB2 sgRNA (PLXNB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5T

Plasmid

#62264

Purpose

expression of A5T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A5NT

Plasmid

#62263

Purpose

expression of A5NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A5NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4T

Plasmid

#62262

Purpose

expression of A4T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A1NT

Plasmid

#62255

Purpose

expression of A1NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1NT

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pF(UG) U6-SYT7 sgRNA 777 HaloTag

Plasmid

#179724

Purpose

U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

sgRNA and HaloTag

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3b

Plasmid

#180433

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3c

Plasmid

#180434

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3d

Plasmid

#180435

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pHiFi Cas9-2×sgRNA (empty, donor)

Plasmid

#162277

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

Depositor

Kazuhisa Nakayama

Article

Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

Insert

HiFi Cas9

Use

CRISPR

Tags

BPNLS and FLAG tag

Expression

Mammalian

Mutation

SpCas9 (R691A)

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT7-A10)-PGKpuro2ABFP-W

Plasmid

#159288

Purpose

Lentiviral vector expressing gRNA targeting KAT7 (gRNA ID. A10)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT7 (ID. A10) (KAT7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b Bxb1 attB38

Plasmid

#242879

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 5541 - 5560 of 16776 results

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