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Plasmid#206159PurposeLuciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorInsert4X PRE TK luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Po-ER Long P2A
Plasmid#164113PurposeDetect the long Peroxisomes-Endoplasmic reticulum contactDepositorInsertSplit YFP Po-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SOX2_P2A_Hygro_Barcode
Plasmid#120480PurposeBarcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DONSON
Plasmid#208797PurposeXenopus laevis His6-Myc-DONSON wild typeDepositorInsertDONSON (donson.L Frog)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107320PurposeExpresses SpCas9 fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 is fused to SaCas9PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLYS5-MCU-Flag
Plasmid#50054PurposeExpression of human MCU in mammalian cellsDepositorInsertMCU-Flag (MCU Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TRC.mKO2
Plasmid#85208PurposeBackbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expressionDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_mTurquoise2
Plasmid#113895PurposeMammalian (inducible) expression of cytosolic mTurquoise2DepositorInsertmonomeric Turquoise2
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1[D157N]
Plasmid#27100DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGFP and S-tagExpressionMammalianMutationD157NPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_EmGFP
Plasmid#113892PurposeMammalian (inducible) expression of cytosolic EmGFPDepositorInsertEmerald GFP
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorInsertAMPK-EV
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHLSec2-FN-YPet
Plasmid#65421PurposeExpresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7DepositorInserthuman plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
UseTagsYPetExpressionMammalianMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH3
Plasmid#168979PurposeAMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…TagsExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA(lib)-puro
Plasmid#119976PurposeLenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only