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Plasmid#50015PurposeMammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertring finger protein 31 (RNF31 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ZmBbm-P2A-ZmWus2-9
Plasmid#197750PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm-P2A-ZmWus2 bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHM6-alphasynuclein-WT
Plasmid#40824DepositorInsertSNCA (SNCA Human)
UseTags6x His and HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDG02583
Plasmid#104129PurposeOptimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)DepositorInsertNEDD8-specific protease 1 (NEDP1)
UseTags14x Histidine tag, Maltose binding protein, and S…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
UseTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_887
Plasmid#201164PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_888
Plasmid#201165PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_886
Plasmid#201162PurposeLentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLSDepositorInsertPuromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFSAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGinB-8X(GGS)-dCas9-FLAG-NLS
Plasmid#81205PurposeCMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminusDepositorInsertpGinB-8X(GGS)-dCas9-FLAG-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-KO
Plasmid#17603PurposeMammalian expression of HA tagged ubiquitin carrying zero lysines (K0)DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationKO: all lysines mutated to argininesPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-mEos2
Plasmid#168515PurposeExpression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rateDepositorInsertmito-mEos2
UseTagsmEos2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-N1
Plasmid#136556PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
UseTagsHis14 and bdNEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
BFP
Plasmid#201383PurposeControl nucleocytoplasmic transport probeDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Lact-C2
Plasmid#74061PurposeFluorescent biosensor for phosphatidylserine.DepositorInsertLactadherin-C2 (MFGE8 Bovine)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
Plasmid#222994PurposeMammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNADepositorInsertHuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaR4 KKR mutations
Plasmid#37199DepositorInsertFokI (fused to RosaR4 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs R4, and SV40 NLSExpressionMammalianMutationI538K, E490K, and H537RPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP720-N1
Plasmid#136560PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720DepositorInsertmiRFP720
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM6-alphasynuclein-A53T
Plasmid#40825DepositorInsertSNCA (SNCA Human)
UseTags6x His and HAExpressionMammalianMutationA53TPromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG mito-GCaMP5G
Plasmid#105009Purposemitochondrial calcium sensorDepositorInsertGCaMP5G
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only