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Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psRaf1
Plasmid#89364PurposeA lenti-viral vector expressing photoswitchable Raf1 in mammalian cellsDepositorInsertpsRaf1 (RAF1 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-Venus in pcDNA6
Plasmid#190802PurposeImaging of α-actinin-4 in live cellsDepositorInsertalpha-actiinin-4-M522-venus (ACTN4 Synthetic, Human)
UseTagsHis, myc, and venusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-2C-NLS in pCSDest
Plasmid#126637PurposeExpresses AsCas12a-2C-NLS in mammalian cellsDepositorInsertAsCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-OVOL2-HA
Plasmid#184737PurposeDoxycycline-inducible lentiviral expression of OVOL2DepositorInsertovo like zinc finger 2 (OVOL2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterTet ONAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-DDB1
Plasmid#19909DepositorInsertDamage-specific DNA binding protein 1 (DDB1 Human)
UseTagsHAExpressionMammalianMutationplease see depositor comment belowPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
OPRD1-DuET
Plasmid#213358PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPRD1 (OPRD1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorInsertRDX (RDX Human)
UseTagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-REDD1-HA
Plasmid#133550PurposeExpresses HA tagged REDD1 in Mammalian cellsDepositorInsertRegulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-NrasG12D
Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
NTSR1-DuET
Plasmid#213356PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertNTSR1 (NTSR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR3-DuET
Plasmid#213378PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertSSTR3 (SSTR3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
YFP-S100A10
Plasmid#107200PurposeExpresses YFP-human S100A10 fusion protein for live cell imagingDepositorInsertS100A10 (S100A10 Human)
UseTagsYellow Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-CAR-GECO1
Plasmid#46022PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
UseTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRGEB-Cas9-VirD2 (PCO)
Plasmid#139802PurposeExpressing Cas9-VirD fusion under ubiquitin promoterDepositorInsertCas9-VirD2
UseTagsCas9-VirD2ExpressionPlantMutationPromoterUbiquitinAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
HCA3-Tango
Plasmid#66397PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertHCA3 (HCAR3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
OPRM1-DuET
Plasmid#213361PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPRM1 (OPRM1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd
Plasmid#135518PurposeMammalian expression of myc-tagged H2-DdDepositorInsertH2-Dd (H2-D1 Mouse)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationC121SPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only