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Plasmid#202208PurposePlasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cellsDepositorInsertCytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
UseLentiviralTagsFLAG and LOV*ExpressionMutationPromoterEf1aAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
His10-tag
Plasmid#55179Purpose10xHis-tagDepositorInsertHis10
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK124
Plasmid#110583PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP25 promoter with lac operator and RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-STAT3-linker-TagGFP2
Plasmid#121427PurposeWT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertSTAT3-linker-TagGFP2
UseTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
JH401: pMVP/Ad/RGD-PK-DEST
Plasmid#121846PurposepMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backboneDepositorTypeEmpty backboneUseAdenoviral; Pmvp gateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Dest_R1-R3
Plasmid#140878Purpose166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.DepositorInsertccdb/CAM
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIb'a' tagExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
cMyc-tag
Plasmid#55183PurposecMyc tagDepositorInsertcMyc tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTK102
Plasmid#110574PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertT7 promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27NPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_WTTS
Plasmid#91233Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-PP7-ccdB
Plasmid#99912PurposeBasic cloning vector for the expression of PP7 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
psgRNA2.02-MS2-ccdB
Plasmid#99911PurposeBasic cloning vector for the expression of MS2 inserted sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSK234
Plasmid#131149Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-FP-GFP
Plasmid#58242PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP markerDepositorInsertattR1-Cm(R)-CcdB-attR2
UseLentiviral and RNAi; Gateway destionation vectorTagsExpressionMammalianMutationPromoternoAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R4 #BGV049
Plasmid#48943PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only