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Plasmid#30495DepositorInsertAhi1 (AHI1 Human)
UseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Map7-Start-1351
Plasmid#46075DepositorInsertMap7-Start-1351 (Map7 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F3bbs kan NNNTGGGCGk
Plasmid#18756DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
IBMc326
Plasmid#161949PurposeContains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20DepositorInsertpSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S65A+T69A+S70A
Plasmid#13616DepositorUseTagsFLAGExpressionMammalianMutationchanged Ser 65, Thr 69 and Ser 70 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSC-NF8-d1-eGFP
Plasmid#122036PurposeAAV vector, NFkappaB-dependent eGFP expressionDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem53-V5
Plasmid#175142PurposeLentiviral expression of mouse Tmem53-V5DepositorInsertTmem53 (Tmem53 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTox-EGFPsite1_NGA-PAM (BPK754)
Plasmid#181747Purposep11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAMDepositorInsertccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
UseSelection plasmidTagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL3
Plasmid#184131PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPges-1 (3.3kb)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOCC94
Plasmid#118912Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
UseTagsMBP, cleavable with 3C protease and eGFP-HIS6ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
VRER variant
Plasmid#138561PurposeExpresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSDDepositorInsertVRER
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingTagsExpressionMutationPromotermNanogAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationH391YPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-hB90
Plasmid#18968DepositorInsertBrevican C-terminus (BCAN Human)
UseTagsExpressionMammalianMutationHuman brevican signal peptide aa 1-23 followed by…PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pIS1 DICER1 long-mut-let-7
Plasmid#21651DepositorInsertDicer 1 (DICER1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant let-7 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only