Addgene

We narrowed to 15,233 results for: LEA

Showing: 5501 - 5520 of 15233 results

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pCMV6-G2-ATP8-V5 (puromycin)

Plasmid

#86852

Purpose

mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Use

Tags

ATP5G2 (G2) mitochondrial targeting signal and V5

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-H63S

Plasmid

#82035

Purpose

Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

H63S MRE11 mutant

Promoter

CMV-tet

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Non-specific sgRNA

Plasmid

#109432

Purpose

MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

Non-specific gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

Plasmid

#62975

Purpose

Expresses HA-tagged rat SOX9 residues 286-507

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Tags

HA (YPYDVPDY)

Expression

Mammalian

Mutation

codes for residues 286-507

Promoter

CMV

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Diacylglycerol Kinase Zeta

Plasmid

#85454

Purpose

expression of Diacylglycerol Kinase Zeta

Depositor

Stephen Gee

Article

Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

Insert

Diacylglycerol Kinase Zeta (DGKZ Human)

Use

Tags

3x HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-VMAT2-WPRE-pA

Plasmid

#39339

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV; R

Tags

Expression

Mutation

Myc tag inserted in the first lumenal loop betwee…

Promoter

Available since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178574

Purpose

Lentiviral vector for CamKIIα::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-SUN1

Plasmid

#213515

Purpose

Expresses human eGFP-SUN1 in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al bioRxiv 2023.05.07.539750

Insert

SUN1 (SUN1 Human)

Use

Tags

Expression

Mammalian

Mutation

SUN1 has a silent mutation on its gene (nucleotid…

Promoter

CMV

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO(ChRger2-TS-YFP)

Plasmid

#127239

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEF Doc2beta WT GFP

Plasmid

#128820

Purpose

To visualize Doc2beta WT in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Fn

Plasmid

#108864

Purpose

FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Type

Empty backbone

Use

CRISPR; Gateway compatible crrna entry clone

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry Codon 59 sgRNA

Plasmid

#109431

Purpose

MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry codon 59 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-p97-E578Q

Plasmid

#85672

Purpose

Expression of human p97 E578Q mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

E578Q

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET26b-SET

Plasmid

#8822

Depositor

Judy Lieberman

Article

Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

Insert

SET (SET Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pSCL.175

Plasmid

#184996

Purpose

Express -Eco1 HEK3 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

HEK3 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-EGFP

Plasmid

#176274

Purpose

Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

EGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

human Synapsin I

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mPCSK8

Plasmid

#122677

Purpose

Expresses mouse PCSK8 in mammalian cells

Depositor

Liming Pei

Article

Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

Insert

Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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