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pDONR223_PYGL_WT_V5
Plasmid#82991PurposeGateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertPYGL (PYGL Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
hTRAF2pLPCX-HA-Flag/P874
Plasmid#20229DepositorInsertTNF receptor-associated factor 2 (TRAF2 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationunmutated NM_021138.3. L replaces M at beginning…PromoterAvailable sinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
NLS-LucWT-GFP
Plasmid#127709PurposeThe expressed ORF encodes nucleus location signal with GFP and WT Luciferase.DepositorInsertLuciferase
UseTagsEGFP and FLAG-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTracer-HV1
Plasmid#24710DepositorInsertHV1 (HVCN1 Human)
UseTagsGFP--NOT a fusion!ExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSExpressionMutationPromoterTRE, PGKAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWAP-HGF
Plasmid#83503PurposeGeneration of transgenic mice overexpressing the HGF under the control of the WAP gene promoterDepositorInsertHGF (Hgf Mouse)
UseMouse TargetingTagsExpressionMutationalso contains beta-globin sequences from pUC198Promotermouse WAP (whey acidic protein)Available sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Lung Cancer Mutant Collection
Plasmid Kit#1000000104PurposePanel of lung adenocarcinoma alleles that have undergone basic, functional screening; full-length human clones (180 mutant and 45 corresponding wild-type) and 82 wild-type experssion controlsDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
CLIMP-63 pcDNA
Plasmid#80977Purposemammalian expression of CLIMP-63DepositorInsertCLIMP-63 (CKAP4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2928
Plasmid#143202PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFIX (NFIX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG (AAV1)
Viral Prep#105537-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCMVTagsNoneAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-CAG-hCAS9-neo
Plasmid#166026PurposeAAVS1 targeting vector carrying CAG-SpCas9-neoDepositorInsertAAVS1-CAGCas9IRESneo
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG.CenpB(FL)-GFP-Halo
Plasmid#176205PurposeCentromeric anchor for TMP-Halo dimerization systemDepositorInsertCenpB-GFP-Halo
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEP-GluA2 TKIT
Plasmid#169442PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16
Plasmid#172746PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-16
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-iRFP720-E2A-Luc
Plasmid#104587PurposeLentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoterDepositorInsertiRFP720-E2A-Luciferase
UseLentiviral and LuciferaseTagsbi-cistronicExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only
