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Showing: 5481 - 5500 of 6157 results

  1. pGen2.1-redCALWY-1

    Plasmid
    #50739
    Purpose
    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-1 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    50739-plasmid-map-sequence-id-76088

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  4. pSU315

    Plasmid
    #169697
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169697-plasmid-map-sequence-id-334518

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  7. pIC291

    Plasmid
    #51403
    Purpose
    Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Article
    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10
    Insert
    Ska1 IMAGE 3909951 (SKA1 Human)
    Use
    Retroviral
    Tags
    EGFP, S tag, and TEV protease site
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51403-plasmid-map-sequence-id-78323

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  10. pSU314

    Plasmid
    #169696
    Purpose
    Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
    Use
    Tags
    1xHA tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169696-plasmid-map-sequence-id-334524

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  15. pENTR-R4R3-stop

    Plasmid
    #113745
    Purpose
    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    stop cassette
    Use
    CRISPR; Gateway entry vector
    Tags
    Expression
    Mutation
    Added 3 stop codons to the original sequence; see…
    Promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113745-plasmid-map-sequence-id-260896

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Showing: 5481 - 5500 of 6157 results