Showing: 5481 - 5500 of 14108 results
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Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4
Plasmid#136611PurposeDox-inducible lentiviral vector expressing mouse Oct4DepositorInsertOct4 (Pou5f1 Mouse)
UseLentiviralTagsExpressionBacterialMutationPromotertetO-miniCMV (dox-inducible)AvailabilityAcademic Institutions and Nonprofits only -
SiC-V2-Cas9G7
Plasmid#133043PurposeDoxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.DepositorInsertTet repressor
UseLentiviralTagsCeruleanExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDarmo.CMVT_v1
Plasmid#133072PurposeEntry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.DepositorTypeEmpty backboneUseEntry vectorTags3C-Flag-TwinStrep and TwinStrep-Flag-3CExpressionMammalianMutationPromoterCMVT (Enhanced CMV)AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-PCP-eGFP
Plasmid#180274Purposeto epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertPCP-eGFP
UseTagseGFPExpressionInsectMutationPromoternanosAvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
UseTagsmCherryExpressionInsectMutationPromoternanosAvailabilityAcademic Institutions and Nonprofits only -
pEGFP/golgin-160
Plasmid#180630PurposeExpression of WT golgin-160DepositorInsertgolgin-160 (GOLGA3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
phFluc-WT
Plasmid#177714PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019DepositorInserthFluc WT
UseTagsExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
UseTagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHS0806 His14-bdSUMO-AwaIscB
Plasmid#176539PurposeBacterial expression of His14-bdSUMO-AwaIscBDepositorInsertAwaIscB
UseCRISPRTags14xHis-bdSUMO tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold
Plasmid#176541Purposehuman U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloningDepositorInsertOgeuIscB omegaRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
UseTagsHis14 tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NES
Plasmid#175008PurposeCytosol-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEZT-GST-SLFN2
Plasmid#174319PurposeExpression of recombinant GST-SLFN2DepositorInsertGST-SLFN2 (Slfn2 Mouse)
UseBacmam, baculovirusTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMal-SLFN2
Plasmid#174322PurposeBacterial expression of MBP-tagged mouse SLFN2DepositorInsertSLFN2 (Slfn2 Mouse)
UseTagsMBPExpressionBacterialMutationPromotertac promoterAvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Raichu-454HRasCT
Plasmid#176029PurposeA FRET biosensor for HRas activityDepositorInsertRaichu-Ras-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-EGFP
Plasmid#176274PurposeViral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-TdTomato
Plasmid#176275PurposeViral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailabilityAcademic Institutions and Nonprofits only
Showing: 5481 - 5500 of 14108 results