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Plasmid#34611Purpose3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tagDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTags2xFlag and mRFPExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-metap2
Plasmid#100512PurposeExpresses HA tagged metap2 in mammalian cellsDepositorInsertMetap2 (METAP2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorInsertE2F1 (E2F1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-GFP
Plasmid#40125DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBUN421
Plasmid#62204PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterTaU3 and UbiAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-H
Plasmid#182281PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.DepositorInsertcAMPFIRE-H (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-TadCBEd-ivt-template
Plasmid#193843PurposeTemplate for in vitro transcription of TadCBEd (with SpCas9)DepositorInsertTadCBEd with SpCas9
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHUE411
Plasmid#62203PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(C163S)
Plasmid#90088PurposeExpresses caspase 3 with C163S to prevent autocatalysis in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationC163SPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NLS
Plasmid#175007PurposeNuclear-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2-NES
Plasmid#175011PurposeCytosol-targeted AktAR2.DepositorInsertAktAR-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKH115_GALNT2-keima
Plasmid#214969PurposeGolgiphagy flux reporter GALNT2-keimaDepositorInsertGALNT2-keima (GALNT2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Foxo1ADA (pCMV5)
Plasmid#12143DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsHA and mycExpressionMammalianMutationPhosphorylation-deficient, also referred to as co…PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmStrawberry-Atg4BC74A
Plasmid#21076DepositorInsertAtg4B(C74A) (Atg4b Mouse)
UseTagsmStrawberryExpressionMammalianMutation220-222 TGC(Cys) is changed to GCA (Ala)PromoterAvailable sinceJune 8, 2009AvailabilityAcademic Institutions and Nonprofits only