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Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-sfGFP
Plasmid#145172PurposeMammalian expression plasmid for sACE2-sfGFP high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsLinker GSGGSGSGG and superfolder GFPExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF1_AE
Plasmid#148569PurposeMammalian Expression of HsGIGYF1DepositorInsertHsGIGYF1 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST
Plasmid#182547PurposeExpresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificityDepositorInsertH5_Vietnam_1203_04_Y161A
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationY161A mutant that confers exclusive NeuAc to NeuG…PromoterCMVAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-plk1-AKAP
Plasmid#132967PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorUseTagsExpressionMammalianMutationC terminus of full-length Plk1 fused to the peric…PromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-eGFP
Plasmid#157734Purposemammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoterDepositorInsertFH-eGFP
UseTagsFlag-His8ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-DroshaS300/302A
Plasmid#62530PurposeSerines 300 and 302 of Drosha are mutated to alaninesDepositorInserthuman Drosha with serines 300 and 302 changed alanines (DROSHA Human)
UseTagsGFPExpressionMammalianMutationchanged serines 300 and 302 to alaninesPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HisGFPHiBiT
Plasmid#188316PurposeExpression of GFP-HiBiT via T7 promoter in TXTLDepositorInsertGFP-HiBiT fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HiBiTGFPHis
Plasmid#188317PurposeExpression of HiBiT-GFP via T7 promoter in TXTLDepositorInsertHiBiT-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST(LSCS)GFPmyc
Plasmid#104451PurposeAutophagy cargo assayDepositorInsertGST-GFP-myc
UseTagsGST and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKK-EGFP-TEV
Plasmid#105776PurposeExpression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)DepositorTypeEmpty backboneUseFlp-in competentTagsEGFP-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL3-GFP
Plasmid#67397PurposeMammalian expression of hArl3 with C-term GFP tagDepositorInsertARL3 (ARL3 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(K179M)
Plasmid#39535DepositorInsertAkt(K179M) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationK179MPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EGFP
Plasmid#14197DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL2-iGFPx6
Plasmid#105251PurposePlasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.DepositorInsertAPL2 portion (APL2 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306GPD-ccdB-EGFP
Plasmid#14188DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
CHAF1B_pcDNA6.2/EmGFP-Bsd
Plasmid#176969PurposeMammalian expression vector encoding CHAF1B and EmGFP-BsdDepositorInsertCHAF1B (CHAF1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAL 25Q+ProGFPp416
Plasmid#15580DepositorInserthtt 25Q + Pro (HTT Human)
UseTagsGFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CADPS KI
Plasmid#131482PurposeEndogenous tagging of CAPS1: N-terminal (amino acid position: S39)DepositorInsertgRNA and GFP donor (Cadps Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only