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Plasmid#32868PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD1A (SETD1A Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
piB-GFP
Plasmid#13844DepositorInsertTATA-GFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_SDHA
Plasmid#177980Purposelentiviral vector expressing Cas9 and a sgRNA targeting SDHADepositorInsertsgRNA targeting SDHA (SDHA Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse Clock delta 19
Plasmid#177312Purposeexpression of mutant mouse clockDepositorInsertmouse clock delta 19
UseTagsExpressionMammalianMutationdeletion from original mClock gene (514-564aa)PromoterCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pRKO
Plasmid#49456PurposeloxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptionsDepositorInsertloxP-kanMX-loxP cassette
UseCre/LoxTagsExpressionBacterialMutationPromoternoneAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR_HNF1A-RE_tBFP_PGK_mCitrine
Plasmid#188387PurposeLentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;DepositorInsertHNF1A-RE_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1 PRF
Plasmid#163317PurposeEncodes for first COVID protein - NSP1, NSP10, NSP11, and fragment of NSP12 fused to nano-luciferaseDepositorInsertNsp1, Nsp10, Nsp11 ,Nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseLuciferaseTagsNanoLucExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLX-UBI-GFP
Plasmid#27245DepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau
Plasmid#46904Purposeexpresses EGFP tagged Tau in mammalian cellsDepositorInsertMAPT (MAPT Human)
UseTagsEGFPExpressionMammalianMutationThis WT htau construct contains human four-repeat…PromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSNG-P2A-Puro
Plasmid#136900PurposeLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)DepositorInsertFNLSNG
UseLentiviralTagsFLAG-NLS and NLSExpressionMutationL1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337RPromoterEF1sAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-sACt-mCherry-NES
Plasmid#138215PurposeEncodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.DepositorInsertsACt-mCherry-NES (Adcy10 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.Luciferase.IRES.GFP
Plasmid#169308PurposeConstitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent proteinDepositorInsertLuciferase
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterSFFVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ITIH4-bio-His
Plasmid#52181PurposeExpresses full-length Inter-alpha-trypsin inhibitor heavy chain H4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITIH4 (ITIH4 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUltra-MDH1-ME1
Plasmid#184465PurposeLentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)DepositorUseLentiviralTagsfused to T2AExpressionMammalianMutationdeletion of stop codonPromoterUbCAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
BRAC/pcDNA3
Plasmid#51967Purposemammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicatorDepositorInsertBRAC
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCD564
Plasmid#153026PurposedxCas9(3.7), MCP-SoxS(R93A/S101A)DepositorInsertdxCas9(3.7), MCP-SoxS(R93A/S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterAvailable sinceAug. 16, 2020AvailabilityAcademic Institutions and Nonprofits only