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Viral Prep#92385-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (#92385). In addition to the viral particles, you will also receive purified Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A plasmid DNA.DepositorPromoterTagsNoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B (Lentiviral Prep)
Viral Prep#92386-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B (#92386). In addition to the viral particles, you will also receive purified Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B plasmid DNA.DepositorPromoterTagsNoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP (AAV1)
Viral Prep#138327-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-synapsin-HaloCaMP1a-EGFP (#138327). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1a-EGFP plasmid DNA. Synapsin-driven HaloCaMP1a, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP (AAV1)
Viral Prep#138328-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-synapsin-HaloCaMP1b-EGFP (#138328). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1b-EGFP plasmid DNA. Synapsin-driven HaloCaMP1b, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP (AAV9)
Viral Prep#138327-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-synapsin-HaloCaMP1a-EGFP (#138327). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1a-EGFP plasmid DNA. Synapsin-driven HaloCaMP1a, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP (AAV9)
Viral Prep#138328-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-synapsin-HaloCaMP1b-EGFP (#138328). In addition to the viral particles, you will also receive purified pAAV-synapsin-HaloCaMP1b-EGFP plasmid DNA. Synapsin-driven HaloCaMP1b, a far-red calcium sensor, fused to EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SIRT7_H187Y
Plasmid#53151Purposeexpression vector for catalytically inactive human Sirtuin7DepositorInsertSirt7 (SIRT7 Human)
UseTagsFLAGExpressionMammalianMutationH187Y, catalytic inactivePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
B2M Bulldozer (gRNA crB2M_13)
Plasmid#84381PurposeThis plasmid expresses a gRNA targeting the first exon of human B2M. Most efficient gRNA targeting B2M, leading to ablation of B2M and MHC class I surface expression in 50% of transfected cellsDepositorInsertB2M Bulldozer crB2M_13 gRNA
UseTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0100
Plasmid#141514PurposeLentiviral vector for overexpressing the NOV transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNOV (CCN3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
RCAS-Neu HA SIINFEKL
Plasmid#125832PurposeRetroviral expression of rat Neu cDNADepositorInsertNeu (Erbb2 Rat)
UseRetroviralTags2x HA and SIINFEKLExpressionMammalianMutationVal to Glu point mutation of codon 664 and trunca…PromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorInsertRev (rev )
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEIGW roGFP2-Orp1
Plasmid#64993PurposeMammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Sox11
Plasmid#120387Purposeover expression of sox11 using infectionDepositorInsertsox11 (Sox11 Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A
Plasmid#28047DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A T22N
Plasmid#28048DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationT22NPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only