Skip to main content
Addgene
Showing: 5401 - 5420 of 14729 results

  1. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

    Enlarge

  5. pASPIre3

    Plasmid
    #154844
    Purpose
    Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154844-plasmid-map-sequence-id-307642

    Enlarge

  9. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid
    #84730
    Purpose
    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Insert
    HyPer2 targeted to nucleus
    Use
    Tags
    NLS (nuclear localisation signal and NLS (nuclear…
    Expression
    Plant
    Mutation
    Promoter
    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84730-plasmid-map-sequence-id-180307

    Enlarge

Showing: 5401 - 5420 of 14729 results