Skip to main content
Addgene
Showing: 5401 - 5420 of 34861 results

  5. pROF148

    Plasmid
    #155330
    Purpose
    Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette. 
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155330-plasmid-map-sequence-id-311083

    Enlarge

  6. pET19-TdTc253

    Plasmid
    #207464
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc253 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207464-plasmid-map-sequence-id-407817

    Enlarge

  7. pET19-TdTc188

    Plasmid
    #207463
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc188 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207463-plasmid-map-sequence-id-407799

    Enlarge

  8. pET19-TdTc180

    Plasmid
    #207462
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc180 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207462-plasmid-map-sequence-id-407791

    Enlarge

  9. pET19-TdTc302

    Plasmid
    #207461
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    TdTc302 (Dntt Mouse)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Promoter
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    207461-plasmid-map-sequence-id-407790

    Enlarge

  10. pCAGGS-SMS1-TEV-HAT

    Plasmid
    #202557
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Use
    Tags
    Histidine-Affinity-tag (HAT) and TEV cleavable si…
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    202557-plasmid-map-sequence-id-399621

    Enlarge

  11. pCAGGS-SMS2-TEV-Twin-Strep

    Plasmid
    #202529
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS2 (SGMS2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TGA)
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202529-plasmid-map-sequence-id-399646

    Enlarge

Showing: 5401 - 5420 of 34861 results