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pME-nlsGal80(opt)

Plasmid

#51618

Purpose

vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80(opt) (GAL80 Budding Yeast)

Use

Vertebrate expression

Tags

NLS

Expression

Mutation

codon optimized

Promoter

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pT3TS-Dest_R1-R3

Plasmid

#140878

Purpose

166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pME-mKOFP2-CAAX

Plasmid

#75155

Purpose

Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-AILR-LplA-ER

Plasmid

#42574

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LplA(AILR) with ER-retention sequence KDEL

Use

Tags

FLAG

Expression

Mutation

Compared to wild-type LplA: tryptophan 37 to alan…

Promoter

CAG

Available since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
BC-A1-002

Plasmid

#78689

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-CAAX

Plasmid

#75153

Purpose

Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mVenus-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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HA-VHL R167W-pRc/CMV

Plasmid

#22082

Depositor

William Kaelin

Article

Kibel et al Science. 1995 Sep 8. 269(5229):1444-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant converting aa 167 from Arginine to T…

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pYPQ239A (dFnCas12a)-TV

Plasmid

#136379

Purpose

CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

dFnCas12a-TV

Use

CRISPR

Tags

Expression

Plant

Mutation

D917A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5a1_Ptet

Plasmid

#179339

Purpose

gRNA Position Vector 5a1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5b1_Ptet

Plasmid

#179341

Purpose

gRNA Position Vector 5b1, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a2_Ptet

Plasmid

#179340

Purpose

gRNA Position Vector 5a2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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C514-E47: attB5r-Halotag-nostop-attB1r

Plasmid

#162963

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK001

Plasmid

#110573

Purpose

Broad-host-range toolkit (BTK) entry vector, with superfolder GFP

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-Arl13b1

Plasmid

#40870

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pFP.R375

Plasmid

#138245

Purpose

Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8

Plasmid

#136376

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBW414hrpL-cIgfp

Plasmid

#61439

Purpose

pSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs34-CI-LVA-t-plam-30gfp-t

Use

Tags

LVA

Expression

Bacterial

Mutation

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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