Skip to main content
Addgene
Showing: 5381 - 5400 of 154129 results

  1. Strain SIJ_488

    Bacterial Strain
    #68246
    Purpose
    E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Bacterial Resistance
    None
    Available since
    Dec. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. MAPK1

    Plasmid
    #73248
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73248-plasmid-map-sequence-id-137205

    Enlarge

  5. hGli1 flag3x

    Plasmid
    #84922
    Purpose
    mammalian expression of Gli1
    Depositor
    Martin Fernandez-Zapico
    Insert
    Gli1 (GLI1 Human)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84922-plasmid-map-sequence-id-422144

    Enlarge

  12. Target Accelerator Lung Cancer Mutant Collection

    Plasmid Kit
    #1000000104
    Purpose
    Panel of lung adenocarcinoma alleles that have undergone basic, functional screening; full-length human clones (180 mutant and 45 corresponding wild-type) and 82 wild-type experssion controls
    Depositor
    Jesse Boehm
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. BPK1520

    Plasmid
    #65777
    Purpose
    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SpCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65777-plasmid-map-sequence-id-316938

    Enlarge

  15. mTagBFP-Nucleus-7

    Plasmid
    #55265
    Purpose
    Localization: Nuclear Localization, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mTagBFP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. TFORF2928

    Plasmid
    #143202
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIX (NFIX Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143202-plasmid-map-sequence-id-396100

    Enlarge

  18. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Insert
    AAVS1-CAGCas9IRESneo
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166026-plasmid-map-sequence-id-328056

    Enlarge

Showing: 5381 - 5400 of 154129 results