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Plasmid#171837PurposeMammalian expression of human WIPI2d K128E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationK128EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dH85E-cs-TEV-STREP
Plasmid#171831PurposeMammalian expression of human WIPI2d H85E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationH85EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dK88E-cs-TEV-STREP
Plasmid#171832PurposeMammalian expression of human WIPI2d K88E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationK88EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dI92E-cs-TEV-STREP
Plasmid#171833PurposeMammalian expression of human WIPI2d I92E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationI92EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dC93E-cs-TEV-STREP
Plasmid#171834PurposeMammalian expression of human WIPI2d C93E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationC93EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-spTorA-GFP(C)
Plasmid#169041PurposeGFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.DepositorInsertH6-spTorA-GFP
UseTags6xHis, TEV cleavage site, and spTorA (signal pept…ExpressionBacterialMutationThe mut3 GFP variant with 5 additional mutations …PromoteraraBADAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-yDrp1-StrepII
Plasmid#174427PurposeExpresses S. pombe Drp1 in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA2
Plasmid#171000PurposeHiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A, R538A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[R537A, R538A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA5
Plasmid#171003PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA6
Plasmid#171004PurposeHiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI, R537A, R538A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp10_SARS-CoV-2
Plasmid#167847PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
UseTags6xHis and AviTagExpressionBacterialMutationPromoterT7Available sinceApril 1, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCri-H6-TEV-GRAB-STREP
Plasmid#128499PurposeExpresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.DepositorInsertGRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
UseTagsHis tag (6x) and Strep tag (2x)ExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-2xTS
Plasmid#117750PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-TO-Casp-rastvTEV-teTVMV2
Plasmid#116098PurposeExpresses a combination of proteases and their reporterDepositorInsertCasp-rastvTEV-teTVMV2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-Halo-TEV-Akt2
Plasmid#87606Purposeexpresses His-halo tagged at the N-terminus of human Akt2 proteinDepositorInsertAKT 2 (AKT2 Human)
UseZebrafish (danio rerio) expressionTagsHis-Halo-TevExpressionMammalianMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C195A
Plasmid#83408Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC195A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C29A
Plasmid#83407Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC29A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only